148897

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 2900..3488 Replicon plasmid pD46-2
Accession NZ_CP048133
Organism Acinetobacter baumannii strain D46

Toxin (Protein)


Gene name brnT Uniprot ID A0A3G6Z3M6
Locus tag KPZ59_RS19250 Protein ID WP_000438826.1
Coordinates 2900..3187 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID N9M5I3
Locus tag KPZ59_RS19255 Protein ID WP_001983304.1
Coordinates 3174..3488 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPZ59_RS19230 (KPZ59_p200001) 139..1089 + 951 WP_001205343.1 replication initiation protein RepM -
KPZ59_RS19235 (KPZ59_p200002) 1082..1657 + 576 WP_001096616.1 plasmid replication DNA-binding protein -
KPZ59_RS19240 (KPZ59_p200003) 1677..1820 + 144 WP_001125246.1 hypothetical protein -
KPZ59_RS19245 (KPZ59_p200004) 2009..2527 - 519 WP_000447193.1 tetratricopeptide repeat protein -
KPZ59_RS19250 (KPZ59_p200005) 2900..3187 + 288 WP_000438826.1 BrnT family toxin Toxin
KPZ59_RS19255 (KPZ59_p200006) 3174..3488 + 315 WP_001983304.1 BrnA antitoxin family protein Antitoxin
KPZ59_RS19260 (KPZ59_p200007) 3616..6027 + 2412 WP_000932953.1 TonB-dependent receptor ZnuD2 -
KPZ59_RS19265 (KPZ59_p200008) 6332..6793 + 462 WP_000761346.1 DIP1984 family protein -
KPZ59_RS19270 (KPZ59_p200009) 7116..7289 - 174 WP_001282484.1 hypothetical protein -
KPZ59_RS19275 (KPZ59_p200010) 7289..7660 - 372 WP_000504218.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..8731 8731


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11303.72 Da        Isoelectric Point: 6.0889

>T148897 WP_000438826.1 NZ_CP048133:2900-3187 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T148897 NZ_CP063898:c4957462-4957052 [Klebsiella variicola]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGTTGGTTCCCTGGCCGCTAAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTGGTGTTTGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12208.84 Da        Isoelectric Point: 7.2026

>AT148897 WP_001983304.1 NZ_CP048133:3174-3488 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT148897 NZ_CP063898:c4957709-4957443 [Klebsiella variicola]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G6Z3M6

Antitoxin

Source ID Structure
AlphaFold DB N9M5I3

References