Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 411653..412290 Replicon chromosome
Accession NZ_CP048117
Organism Bacillus sp. SB49

Toxin (Protein)


Gene name pemK Uniprot ID L5NDX6
Locus tag M662_RS02175 Protein ID WP_008632962.1
Coordinates 411940..412290 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A7Z2SU23
Locus tag M662_RS02170 Protein ID WP_026576976.1
Coordinates 411653..411934 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M662_RS02150 407220..407579 + 360 WP_026576978.1 holo-ACP synthase -
M662_RS02155 407648..409141 + 1494 WP_026576977.1 NAD(P)H-hydrate dehydratase -
M662_RS02160 409187..410209 + 1023 WP_008632968.1 outer membrane lipoprotein carrier protein LolA -
M662_RS02165 410354..411502 + 1149 WP_008632967.1 alanine racemase -
M662_RS02170 411653..411934 + 282 WP_026576976.1 hypothetical protein Antitoxin
M662_RS02175 411940..412290 + 351 WP_008632962.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M662_RS02180 412506..413375 + 870 WP_008632960.1 RsbT co-antagonist protein RsbRA -
M662_RS02185 413372..413737 + 366 WP_193743854.1 STAS domain-containing protein -
M662_RS02190 413740..414141 + 402 WP_008632951.1 anti-sigma regulatory factor -
M662_RS02195 414156..415166 + 1011 WP_026576975.1 PP2C family protein-serine/threonine phosphatase -
M662_RS02200 415232..415561 + 330 WP_008632949.1 STAS domain-containing protein -
M662_RS02205 415561..416040 + 480 WP_201029326.1 anti-sigma B factor RsbW -
M662_RS02210 416012..416791 + 780 WP_026576974.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12945.02 Da        Isoelectric Point: 7.0122

>T148895 WP_008632962.1 NZ_CP048117:411940-412290 [Bacillus sp. SB49]
VIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAEKYGFERNSVILLEQI
RTIDKQRLTDKITQLDEPMMQQVNKSLQISLGLIDF

Download         Length: 351 bp

>T148895 NZ_CP063898:c3863882-3863780 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10837.34 Da        Isoelectric Point: 4.5577

>AT148895 WP_026576976.1 NZ_CP048117:411653-411934 [Bacillus sp. SB49]
MVLSDSMQEIVIRIPDNLLNEVDGFIQLENSDRSDFMCQATKMYLREKKQRHIRESMRRGYMEMAKINLNIASEAFQAEE
EADHTLEQLVSGV

Download         Length: 282 bp

>AT148895 NZ_CP063898:3863774-3863918 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z2SU39

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z2SU23

References