Detailed information of TA system TA148892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1869..2422
• Replicon: plasmid unnamed6
• Accession: NZ_CP048112
• Organism: Klebsiella michiganensis strain BD177

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: GW952_RS32515
• Protein ID: WP_162123015.1
• Coordinates: 2108..2422 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A6P1V7Y5
• Locus tag: GW952_RS32510
• Protein ID: WP_025861853.1
• Coordinates: 1869..2105 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GW952_RS32500	1..348	+	348	WP_162123013.1	hypothetical protein	-
GW952_RS32505	1160..1729	+	570	WP_162123014.1	hypothetical protein	-
GW952_RS32510	1869..2105	+	237	WP_025861853.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GW952_RS32515	2108..2422	+	315	WP_162123015.1	CcdB family protein	Toxin
GW952_RS32835	2463..2627	+	165	WP_202929187.1	hypothetical protein	-
GW952_RS32840	2643..2735	+	93	Protein_5	XRE family transcriptional regulator	-
GW952_RS32520	2922..3479	+	558	WP_162123016.1	nucleotidyltransferase family protein	-
GW952_RS32525	4017..4799	+	783	WP_162123017.1	sprT domain-containing protein	-
GW952_RS32530	4919..5566	+	648	WP_162123018.1	ParA family protein	-
GW952_RS32535	5607..5840	+	234	WP_004178129.1	hypothetical protein	-
GW952_RS32540	5878..6219	-	342	WP_011172022.1	hypothetical protein	-
GW952_RS32545	6280..6753	-	474	WP_162123019.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..23848	23848

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11567.45 Da
• Isoelectric Point: 5.8036

>T148892 WP_162123015.1 NZ_CP048112:2108-2422 [Klebsiella michiganensis]
MQFTVYNNTGKSAVYPLLLDVTSDIIGQLNRRIVIPLLPVEKYPGSTRPERLVPLVRLTDDKEYAVMTHEMASIPARSLG
AVFCDASPYRTEVKAAIDFLLDGI

Nucleotide

• Length: 315 bp

>T148892 NZ_CP063898:c1557394-1557176 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCTGACCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCCACATCAGTCTGGAAGTTTATCCGCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8907.84 Da
• Isoelectric Point: 4.8040

>AT148892 WP_025861853.1 NZ_CP048112:1869-2105 [Klebsiella michiganensis]
MATTQRATQSVTMTVERALLSRAREAGINLSATLTAALDAELRQYEAKKWQEENNEALEALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT148892 NZ_CP063898:c1557795-1557421 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A6P1V7Y5