Detailed information of TA system TA148891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 154437..154971
• Replicon: plasmid unnamed3
• Accession: NZ_CP048111
• Organism: Klebsiella michiganensis strain BD177

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6P1V8P2
• Locus tag: GW952_RS32440
• Protein ID: WP_000143805.1
• Coordinates: 154437..154727 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GW952_RS32445
• Protein ID: WP_162122997.1
• Coordinates: 154717..154971 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GW952_RS32400	149590..149859	+	270	WP_162122990.1	holin	-
GW952_RS32405	149837..150334	+	498	WP_202929185.1	lysozyme	-
GW952_RS32410	150331..150702	+	372	WP_162122992.1	hypothetical protein	-
GW952_RS32415	151030..151365	-	336	WP_162122993.1	hypothetical protein	-
GW952_RS32420	151454..151750	+	297	WP_202929186.1	hemagglutinin	-
GW952_RS32425	151747..152103	+	357	WP_162122995.1	DUF596 domain-containing protein	-
GW952_RS32430	152433..152588	-	156	Protein_171	IS30 family transposase	-
GW952_RS32435	152894..153916	+	1023	WP_162122996.1	IS110 family transposase	-
GW952_RS32440	154437..154727	-	291	WP_000143805.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GW952_RS32445	154717..154971	-	255	WP_162122997.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GW952_RS32450	155128..155838	-	711	WP_162122998.1	tyrosine-type recombinase/integrase	-
GW952_RS32455	155925..156320	-	396	WP_032672763.1	hypothetical protein	-
GW952_RS32460	157090..157569	+	480	WP_162122999.1	hypothetical protein	-
GW952_RS32465	157664..158704	+	1041	WP_162122276.1	IS481 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..164510	164510
	-	inside	IScluster/Tn	-	-	152894..162445	9551

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11463.41 Da
• Isoelectric Point: 10.2689

>T148891 WP_000143805.1 NZ_CP048111:c154727-154437 [Klebsiella michiganensis]
MTFNVEFDERAFKEWNKLEKTIREQFKKKLKKLQQNPYVESARLHGDLAGCFKIKLRASGFRLIYKVIDDEIVIWVVAVG
KREDEKAYETARKRLL

Nucleotide

• Length: 291 bp

>T148891 NZ_CP063898:680633-681550 [Klebsiella variicola]
ATGGATATACAGGCGCCCTATTATCGGCAGGTTGCTCTGTTAATGCAGGTTTTACCCTATGTTGCCGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACGGCAATCAATCTCTTTATCCGTGATTTCCCACGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAACGATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATAAACGCTGTATTACAAGCTAATCGTTCCGATGAGATGCGTGTCGTTGTTACTACCGATAGCGCACAAATAAA
GATAGAGGTCTCACCGGTCGCACGGGGAACATTATATCCACCACAGGAGCGAGAGGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCGGTGGTTTCGTTGCCCGATCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGATGTGAAATTACTTCTTGATGCGCAGGAGCTTAACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTTAGCCATAATCGGCCTCTGGCTGAAGTGCTTAGTCCTCGCTGGAAAGATATTGCTGAACCCTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCTACAGGATGATTGATGCGTTAAAATCC
TGTTTCACGCAACAGGATATCGATTTTTTGCTGTCATTTAAACGAGGCGAACCAGACTGGCGGTTAGTGCCGGAAACACG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCGTTAG
ATAAACTGGAAAAGGTGTTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9694.01 Da
• Isoelectric Point: 4.4137

>AT148891 WP_162122997.1 NZ_CP048111:c154971-154717 [Klebsiella michiganensis]
MSTIQIRVDDELKKDAYQAFEKLNLSPSDALRLFLRYVAENEKLPFAEVSVMVSDDDEDADILAVVRERLKNPARRIRVN
VDDL

Nucleotide

• Length: 255 bp

>AT148891 NZ_CP063898:679869-680627 [Klebsiella variicola]
ATGTCATCAAAATTAAACTGGCTGCTGCAAAACAGTGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCGGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACACGCAAAGTGGCTGGTTAAAAAAACGAGGCAACGGCGTATACGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTAGCCGCTCCGGTGTATGTCGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGTCGTTCACACTATCTACAGCTCAAACAAAACCAGTGCTGGTTGAGTATGGAAAATAAAGCGCT
GCTGCCAAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGCAAAAACTGCCGGTGCTGGATGATA
AATACCGCGTAACGCTTGATGTAAAAGGGAAAAGACTCACCGGCAGTGCGCCGGAACTGGCTGCTTACGAGTTGTTGAGC
GCGGTACCGGGGACCCTCTCATTTAACCACGCGGCAGAGCTTTTCCAGGGACTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTGCTTTCTGTCAGCCAGTCCGTGCAGACCAAGCGTTTGTATCTCTTTTTCGCCAGTTTTTACGAGCATGGTTGGC
TCAGACGAATCGATAGCCAAAAAATCGATCTGGGTGCGGGTAAGCGGCAAATCGTGGTAAATGGCAAGTTCAATGCTCAA
TATCAAATCACTGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A6P1V8P2