Detailed information of TA system TA148889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 8257..8776
• Replicon: plasmid unnamed3
• Accession: NZ_CP048111
• Organism: Klebsiella michiganensis strain BD177

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A6P1V779
• Locus tag: GW952_RS31625
• Protein ID: WP_050880437.1
• Coordinates: 8492..8776 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A6P1V8C3
• Locus tag: GW952_RS31620
• Protein ID: WP_000124732.1
• Coordinates: 8257..8499 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GW952_RS31590	4527..5210	+	684	WP_162123002.1	IS6 family transposase	-
GW952_RS31595	5607..5798	-	192	WP_079959158.1	ribbon-helix-helix domain-containing protein	-
GW952_RS31600	5960..6283	-	324	WP_162122904.1	CcdB family protein	-
GW952_RS31605	6283..6531	-	249	WP_079959175.1	type II toxin-antitoxin system CcdA family antitoxin	-
GW952_RS31610	6800..7495	+	696	WP_162122905.1	IS6 family transposase	-
GW952_RS31615	7550..7918	+	369	Protein_9	transposase	-
GW952_RS31620	8257..8499	+	243	WP_000124732.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
GW952_RS31625	8492..8776	+	285	WP_050880437.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GW952_RS31630	8899..9592	+	694	WP_162122906.1	IS6 family transposase	-
GW952_RS31635	9656..10351	+	696	WP_162122905.1	IS6 family transposase	-
GW952_RS31640	10959..11570	+	612	WP_162122907.1	DUF2726 domain-containing protein	-
GW952_RS31645	11844..12518	+	675	WP_162122908.1	hypothetical protein	-
GW952_RS31650	12595..12954	+	360	WP_162122909.1	hypothetical protein	-
GW952_RS31655	13017..13415	+	399	WP_162122910.1	hypothetical protein	-
GW952_RS31660	13412..13720	+	309	WP_162122911.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..164510	164510
	-	inside	IScluster/Tn	-	-	4371..10351	5980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-88)

Antitoxin

• Antitoxin ParD (1-64)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11017.58 Da
• Isoelectric Point: 6.2111

>T148889 WP_050880437.1 NZ_CP048111:8492-8776 [Klebsiella michiganensis]
VYKLTERAVEDFAGIYDYTLLKFGEAQADHYTDALEAFFETLAGMPDMGRDYHAVPGVMRIEFQRHAIFYTVRDTDILIA
RILHQQMNHKRHLL

Nucleotide

• Length: 285 bp

>T148889 NZ_CP063897:c147585-147103 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8875.01 Da
• Isoelectric Point: 7.0130

>AT148889 WP_000124732.1 NZ_CP048111:8257-8499 [Klebsiella michiganensis]
MARTTSVTIGEQLDSFISRLIDSGRYGSASEVMRSALRLLEQQETNDEVIRQAVIAGLESGESSLSLRDIAAQRKLRHRV

Nucleotide

• Length: 243 bp

>AT148889 NZ_CP063897:c147839-147573 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A6P1V779

Antitoxin

• AlphaFold DB: A0A6P1V8C3