Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 272138..272889 Replicon plasmid unnamed1
Accession NZ_CP048109
Organism Klebsiella michiganensis strain BD177

Toxin (Protein)


Gene name ataT Uniprot ID A0A6P1V716
Locus tag GW952_RS30515 Protein ID WP_048978473.1
Coordinates 272407..272889 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A6P1V8A4
Locus tag GW952_RS30510 Protein ID WP_048978474.1
Coordinates 272138..272416 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GW952_RS30490 267838..268773 + 936 WP_004026564.1 omptin family outer membrane protease Kop -
GW952_RS30495 269246..269728 + 483 WP_186338435.1 hypothetical protein -
GW952_RS30500 269972..270286 - 315 WP_048291862.1 hypothetical protein -
GW952_RS30505 271294..271830 - 537 WP_048978475.1 hypothetical protein -
GW952_RS30510 272138..272416 + 279 WP_048978474.1 DUF1778 domain-containing protein Antitoxin
GW952_RS30515 272407..272889 + 483 WP_048978473.1 GNAT family N-acetyltransferase Toxin
GW952_RS30520 272927..273331 + 405 WP_117042408.1 DUF2251 domain-containing protein -
GW952_RS30525 274099..274970 + 872 Protein_291 ParA family protein -
GW952_RS30530 274967..275347 + 381 WP_162122145.1 hypothetical protein -
GW952_RS30535 275344..275586 + 243 WP_142732979.1 hypothetical protein -
GW952_RS30540 275596..276966 + 1371 WP_162122432.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..281962 281962


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(44-144)

Antitoxin

Vibrio phage ICP1, Orf50

(10-86)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17763.69 Da        Isoelectric Point: 9.4946

>T148886 WP_048978473.1 NZ_CP048109:272407-272889 [Klebsiella michiganensis]
MGLRPPEPLTPEHNIAEFCCQDQVLSEWLKKKALKNHRTGISRVFVVCAENTNRVIAYYCLASGSVHRNTVPGAYRRNAP
EALPVIVLGRLAVDAAWARKGLGAALLKDAIYRTEHIAIQVGVRALLVHALNDEVREFYTKFGFEPSIANALTLLFPIKT

Download         Length: 483 bp

>T148886 NZ_CP063896:3224300-3224710 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCTCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GACTGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10694.21 Da        Isoelectric Point: 4.5038

>AT148886 WP_048978474.1 NZ_CP048109:272138-272416 [Klebsiella michiganensis]
MKPDIREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDNQFNAFVEELDAPMTPERQAR
IDNLMSRKSPWD

Download         Length: 279 bp

>AT148886 NZ_CP063896:3224053-3224319 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCTCGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TATTCAACTGGCTGATGAACCATGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6P1V716

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V8A4

References