Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 139020..139930 Replicon plasmid unnamed1
Accession NZ_CP048109
Organism Klebsiella michiganensis strain BD177

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag GW952_RS29815 Protein ID WP_004144067.1
Coordinates 139020..139490 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag GW952_RS29820 Protein ID WP_004026357.1
Coordinates 139487..139930 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GW952_RS29785 134389..134685 - 297 WP_099745245.1 hydrogenase expression/formation protein HypD -
GW952_RS29790 135012..135389 + 378 WP_004144074.1 hypothetical protein -
GW952_RS32820 135587..136561 - 975 WP_182952497.1 Hok/Gef family protein -
GW952_RS32825 136951..137118 + 168 WP_004117900.1 hypothetical protein -
GW952_RS29800 137148..137390 + 243 WP_086528906.1 hypothetical protein -
GW952_RS29805 137769..137927 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
GW952_RS29810 138650..138910 + 261 WP_004026352.1 hypothetical protein -
GW952_RS29815 139020..139490 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
GW952_RS29820 139487..139930 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
GW952_RS29825 140031..141302 - 1272 WP_004026359.1 translesion error-prone DNA polymerase V subunit UmuC -
GW952_RS29830 141302..141724 - 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
GW952_RS29835 142078..143265 - 1188 WP_101415803.1 transposase -
GW952_RS29840 143322..143729 + 408 WP_101415802.1 IS200/IS605 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..281962 281962


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T148885 WP_004144067.1 NZ_CP048109:c139490-139020 [Klebsiella michiganensis]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T148885 NZ_CP063896:c1863321-1863040 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGGGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT148885 WP_004026357.1 NZ_CP048109:c139930-139487 [Klebsiella michiganensis]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT148885 NZ_CP063896:c1863553-1863311 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGGCTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGAGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References