Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 109039..109616 Replicon plasmid unnamed1
Accession NZ_CP048109
Organism Klebsiella michiganensis strain BD177

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag GW952_RS29665 Protein ID WP_142983937.1
Coordinates 109284..109616 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag GW952_RS29660 Protein ID WP_142983938.1
Coordinates 109039..109284 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GW952_RS29630 104334..104996 - 663 WP_142983942.1 MFS transporter -
GW952_RS29635 105069..105407 + 339 WP_142983941.1 VOC family protein -
GW952_RS29640 106047..106682 + 636 WP_004117933.1 hypothetical protein -
GW952_RS29650 107826..108011 - 186 WP_162122729.1 hypothetical protein -
GW952_RS29655 108253..108864 - 612 WP_142983939.1 hypothetical protein -
GW952_RS29660 109039..109284 + 246 WP_142983938.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GW952_RS29665 109284..109616 + 333 WP_142983937.1 endoribonuclease MazF Toxin
GW952_RS29670 109753..109938 + 186 WP_142983936.1 hypothetical protein -
GW952_RS29675 110000..110371 - 372 WP_142983935.1 hypothetical protein -
GW952_RS29680 110905..111168 + 264 WP_074427173.1 hypothetical protein -
GW952_RS29685 111259..111564 + 306 WP_142983934.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..281962 281962
- flank IS/Tn - - 107505..107780 275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11927.78 Da        Isoelectric Point: 8.2685

>T148884 WP_142983937.1 NZ_CP048109:109284-109616 [Klebsiella michiganensis]
MVKRYVPDAGDLIWIDFDPVAGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTQIKGYPFEVTLSGSKEGVALSDQVTCV
DWRARKVSKKDAVNSTELAEIRAKAKALIG

Download         Length: 333 bp

>T148884 NZ_CP063896:1149158-1149376 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8686.00 Da        Isoelectric Point: 4.7045

>AT148884 WP_142983938.1 NZ_CP048109:109039-109284 [Klebsiella michiganensis]
MAQAIVKKWGNSPSVRLPVAVMKAASLNVDDTVDIEVEDGRIIIVPVRQKEYSLDALLAGISTDNLHGKADFGPAEGKEL
L

Download         Length: 246 bp

>AT148884 NZ_CP063896:1148757-1149131 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References