Detailed information of TA system TA148884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 109039..109616
• Replicon: plasmid unnamed1
• Accession: NZ_CP048109
• Organism: Klebsiella michiganensis strain BD177

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GW952_RS29665
• Protein ID: WP_142983937.1
• Coordinates: 109284..109616 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GW952_RS29660
• Protein ID: WP_142983938.1
• Coordinates: 109039..109284 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GW952_RS29630	104334..104996	-	663	WP_142983942.1	MFS transporter	-
GW952_RS29635	105069..105407	+	339	WP_142983941.1	VOC family protein	-
GW952_RS29640	106047..106682	+	636	WP_004117933.1	hypothetical protein	-
GW952_RS29650	107826..108011	-	186	WP_162122729.1	hypothetical protein	-
GW952_RS29655	108253..108864	-	612	WP_142983939.1	hypothetical protein	-
GW952_RS29660	109039..109284	+	246	WP_142983938.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GW952_RS29665	109284..109616	+	333	WP_142983937.1	endoribonuclease MazF	Toxin
GW952_RS29670	109753..109938	+	186	WP_142983936.1	hypothetical protein	-
GW952_RS29675	110000..110371	-	372	WP_142983935.1	hypothetical protein	-
GW952_RS29680	110905..111168	+	264	WP_074427173.1	hypothetical protein	-
GW952_RS29685	111259..111564	+	306	WP_142983934.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..281962	281962
	-	flank	IS/Tn	-	-	107505..107780	275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.78 Da
• Isoelectric Point: 8.2685

>T148884 WP_142983937.1 NZ_CP048109:109284-109616 [Klebsiella michiganensis]
MVKRYVPDAGDLIWIDFDPVAGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTQIKGYPFEVTLSGSKEGVALSDQVTCV
DWRARKVSKKDAVNSTELAEIRAKAKALIG

Nucleotide

• Length: 333 bp

>T148884 NZ_CP063896:1149158-1149376 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8686.00 Da
• Isoelectric Point: 4.7045

>AT148884 WP_142983938.1 NZ_CP048109:109039-109284 [Klebsiella michiganensis]
MAQAIVKKWGNSPSVRLPVAVMKAASLNVDDTVDIEVEDGRIIIVPVRQKEYSLDALLAGISTDNLHGKADFGPAEGKEL
L

Nucleotide

• Length: 246 bp

>AT148884 NZ_CP063896:1148757-1149131 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG