Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 5574693..5575350 Replicon chromosome
Accession NZ_CP048108
Organism Klebsiella michiganensis strain BD177

Toxin (Protein)


Gene name cptA Uniprot ID A0A6P1V2U3
Locus tag GW952_RS26375 Protein ID WP_046876661.1
Coordinates 5574940..5575350 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag GW952_RS26370 Protein ID WP_004124953.1
Coordinates 5574693..5574959 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GW952_RS26355 5569929..5570354 - 426 WP_160741262.1 PTS sugar transporter subunit IIA -
GW952_RS26360 5570475..5573273 - 2799 WP_162122492.1 transcriptional regulator DagR -
GW952_RS26365 5573465..5574448 - 984 WP_160741260.1 tRNA-modifying protein YgfZ -
GW952_RS26370 5574693..5574959 + 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
GW952_RS26375 5574940..5575350 + 411 WP_046876661.1 protein YgfX Toxin
GW952_RS26380 5575359..5575880 - 522 WP_014226792.1 flavodoxin FldB -
GW952_RS26385 5576002..5576898 + 897 WP_162122493.1 site-specific tyrosine recombinase XerD -
GW952_RS26390 5576921..5577634 + 714 WP_160741258.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GW952_RS26395 5577640..5579373 + 1734 WP_160741257.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16074.91 Da        Isoelectric Point: 10.9455

>T148883 WP_046876661.1 NZ_CP048108:5574940-5575350 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLVLPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Download         Length: 411 bp

>T148883 NZ_CP063891:c167937-167494 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT148883 WP_004124953.1 NZ_CP048108:5574693-5574959 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT148883 NZ_CP063891:c168164-167934 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6P1V2U3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References