Detailed information of TA system TA148880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3084677..3085296
• Replicon: chromosome
• Accession: NZ_CP048108
• Organism: Klebsiella michiganensis strain BD177

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H3N9D8
• Locus tag: GW952_RS14520
• Protein ID: WP_004099646.1
• Coordinates: 3085078..3085296 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: GW952_RS14515
• Protein ID: WP_160741767.1
• Coordinates: 3084677..3085051 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GW952_RS14505	3079832..3081025	+	1194	WP_160741765.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
GW952_RS14510	3081048..3084194	+	3147	WP_160741766.1	multidrug efflux RND transporter permease subunit AcrB	-
GW952_RS14515	3084677..3085051	+	375	WP_160741767.1	Hha toxicity modulator TomB	Antitoxin
GW952_RS14520	3085078..3085296	+	219	WP_004099646.1	hemolysin expression modulator Hha	Toxin
GW952_RS14525	3085459..3086025	+	567	WP_162121912.1	maltose O-acetyltransferase	-
GW952_RS14530	3086152..3086622	+	471	WP_160741768.1	YlaC family protein	-
GW952_RS14535	3086597..3088051	-	1455	WP_162122667.1	PLP-dependent aminotransferase family protein	-
GW952_RS14540	3088153..3088851	+	699	WP_160741769.1	GNAT family N-acetyltransferase	-
GW952_RS14545	3088848..3088988	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
GW952_RS14550	3088988..3089251	-	264	WP_004848323.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T148880 WP_004099646.1 NZ_CP048108:3085078-3085296 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Nucleotide

• Length: 219 bp

>T148880 NZ_CP048108:3085078-3085296 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCTGAGC
TGACGATGAATAAACTGTATGACAAGATCCCTCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14378.14 Da
• Isoelectric Point: 4.8989

>AT148880 WP_160741767.1 NZ_CP048108:3084677-3085051 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSVDLQKWRKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT148880 NZ_CP048108:3084677-3085051 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAGCTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGATTACCCAGATTGATGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGTAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0H3H713