Detailed information of TA system TA148868

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4575344..4576179
• Replicon: chromosome
• Accession: NZ_CP048107
• Organism: Escherichia coli strain 201609

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: GWK05_RS21815
• Protein ID: WP_072721742.1
• Coordinates: 4575344..4575721 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7W3AJG7
• Locus tag: GWK05_RS21820
• Protein ID: WP_001389198.1
• Coordinates: 4575811..4576179 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK05_RS21795	4571697..4573235	-	1539	Protein_4266	lysine decarboxylation/transport transcriptional activator CadC	-
GWK05_RS21800	4573984..4574553	-	570	WP_001290247.1	DUF4942 domain-containing protein	-
GWK05_RS21805	4574650..4574847	-	198	WP_000839281.1	DUF957 domain-containing protein	-
GWK05_RS21810	4574859..4575347	-	489	WP_072647689.1	hypothetical protein	-
GWK05_RS21815	4575344..4575721	-	378	WP_072721742.1	TA system toxin CbtA family protein	Toxin
GWK05_RS21820	4575811..4576179	-	369	WP_001389198.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GWK05_RS21825	4576342..4576563	-	222	WP_000692303.1	DUF987 domain-containing protein	-
GWK05_RS21830	4576626..4577102	-	477	WP_001186710.1	RadC family protein	-
GWK05_RS21835	4577114..4577593	-	480	WP_000860065.1	antirestriction protein	-
GWK05_RS21840	4577675..4578493	-	819	WP_001234631.1	DUF945 domain-containing protein	-
GWK05_RS21845	4578592..4578825	-	234	WP_001278287.1	DUF905 family protein	-
GWK05_RS21850	4578831..4579508	-	678	WP_001097305.1	hypothetical protein	-
GWK05_RS21855	4579656..4580336	-	681	WP_001593697.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14074.15 Da
• Isoelectric Point: 8.2904

>T148868 WP_072721742.1 NZ_CP048107:c4575721-4575344 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
STCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T148868 NZ_CP063889:3381845-3382255 [Klebsiella variicola]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGTTGGTTCCCTGGCCGCTAAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTAGTGTTTGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13610.39 Da
• Isoelectric Point: 6.3159

>AT148868 WP_001389198.1 NZ_CP048107:c4576179-4575811 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT148868 NZ_CP063889:3381598-3381864 [Klebsiella variicola]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7W3AJG7