Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4250859..4251409 Replicon chromosome
Accession NZ_CP048107
Organism Escherichia coli strain 201609

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag GWK05_RS20395 Protein ID WP_001160966.1
Coordinates 4250859..4251173 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag GWK05_RS20400 Protein ID WP_000125568.1
Coordinates 4251176..4251409 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWK05_RS20370 4246534..4247820 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
GWK05_RS20375 4247820..4248809 + 990 WP_000241224.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
GWK05_RS20380 4248806..4249627 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
GWK05_RS20385 4249630..4250007 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
GWK05_RS20390 4250014..4250862 + 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
GWK05_RS20395 4250859..4251173 - 315 WP_001160966.1 CcdB family protein Toxin
GWK05_RS20400 4251176..4251409 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
GWK05_RS20405 4251495..4251974 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
GWK05_RS20410 4252166..4254028 - 1863 WP_022645165.1 glutathione-regulated potassium-efflux system protein KefC -
GWK05_RS20415 4254021..4254551 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
GWK05_RS20420 4254659..4255990 - 1332 WP_001183201.1 MFS transporter -
GWK05_RS20425 4256048..4256335 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T148866 WP_001160966.1 NZ_CP048107:c4251173-4250859 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T148866 NZ_CP063889:2879313-2879681 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT148866 WP_000125568.1 NZ_CP048107:c4251409-4251176 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT148866 NZ_CP063889:2879095-2879316 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGACAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTATACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAATTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATTATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References