Detailed information of TA system TA148862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3433717..3434422
• Replicon: chromosome
• Accession: NZ_CP048107
• Organism: Escherichia coli strain 201609

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: GWK05_RS16575
• Protein ID: WP_000539521.1
• Coordinates: 3433717..3434103 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GWK05_RS16580
• Protein ID: WP_001280945.1
• Coordinates: 3434093..3434422 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK05_RS16555	3429721..3430347	+	627	WP_001295292.1	glutathione S-transferase GstB	-
GWK05_RS16560	3430344..3431459	-	1116	WP_000554964.1	aldose sugar dehydrogenase YliI	-
GWK05_RS16565	3431570..3431953	-	384	WP_072645479.1	biofilm formation regulator BssR	-
GWK05_RS16570	3432166..3433491	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
GWK05_RS16575	3433717..3434103	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GWK05_RS16580	3434093..3434422	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
GWK05_RS16585	3434492..3435819	-	1328	Protein_3250	diguanylate cyclase	-
GWK05_RS16590	3435827..3438175	-	2349	WP_162107937.1	EAL domain-containing protein	-
GWK05_RS16595	3438352..3439263	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T148862 WP_000539521.1 NZ_CP048107:3433717-3434103 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T148862 NZ_CP063889:c1157210-1156893 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTATGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT148862 WP_001280945.1 NZ_CP048107:3434093-3434422 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT148862 NZ_CP063889:c1156900-1156613 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6