Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1055206..1055860 Replicon chromosome
Accession NZ_CP048107
Organism Escherichia coli strain 201609

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag GWK05_RS05025 Protein ID WP_000244781.1
Coordinates 1055453..1055860 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GWK05_RS05020 Protein ID WP_000354046.1
Coordinates 1055206..1055472 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWK05_RS05000 1051284..1052717 - 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -
GWK05_RS05005 1052762..1053073 + 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
GWK05_RS05010 1053237..1053896 + 660 WP_000250269.1 hemolysin III family protein -
GWK05_RS05015 1053973..1054953 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
GWK05_RS05020 1055206..1055472 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GWK05_RS05025 1055453..1055860 + 408 WP_000244781.1 protein YgfX Toxin
GWK05_RS05030 1055900..1056421 - 522 WP_001055874.1 flavodoxin FldB -
GWK05_RS05035 1056533..1057429 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GWK05_RS05040 1057454..1058164 + 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GWK05_RS05045 1058170..1059903 + 1734 WP_000813233.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T148843 WP_000244781.1 NZ_CP048107:1055453-1055860 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T148843 NZ_CP063885:c1101168-1100758 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT148843 WP_000354046.1 NZ_CP048107:1055206-1055472 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT148843 NZ_CP063885:c1101415-1101149 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References