Detailed information of TA system TA148842

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 807980..808821
• Replicon: chromosome
• Accession: NZ_CP048107
• Organism: Escherichia coli strain 201609

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1M0WJM1
• Locus tag: GWK05_RS03835
• Protein ID: WP_000854822.1
• Coordinates: 808438..808821 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GWK05_RS03830
• Protein ID: WP_048943268.1
• Coordinates: 807980..808348 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK05_RS03790	803463..804002	+	540	WP_162107836.1	DUF4339 domain-containing protein	-
GWK05_RS03795	804272..804685	+	414	WP_000789536.1	hypothetical protein	-
GWK05_RS03800	804824..805002	-	179	Protein_746	hypothetical protein	-
GWK05_RS03805	805096..805914	+	819	WP_048943269.1	DUF945 domain-containing protein	-
GWK05_RS03810	806005..806490	+	486	WP_000214317.1	antirestriction protein	-
GWK05_RS03815	806505..806981	+	477	WP_001384029.1	RadC family protein	-
GWK05_RS03820	807050..807271	+	222	WP_000692353.1	DUF987 domain-containing protein	-
GWK05_RS03825	807286..807930	+	645	WP_029396485.1	hypothetical protein	-
GWK05_RS03830	807980..808348	+	369	WP_048943268.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GWK05_RS03835	808438..808821	+	384	WP_000854822.1	type IV toxin-antitoxin system toxin CbtA	Toxin
GWK05_RS03840	808812..809300	+	489	WP_001177592.1	hypothetical protein	-
GWK05_RS03845	809312..809509	+	198	WP_162107837.1	DUF957 domain-containing protein	-
GWK05_RS03850	809606..810451	+	846	WP_001280435.1	DUF4942 domain-containing protein	-
GWK05_RS03860	810750..811256	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
GWK05_RS03865	811335..813176	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14295.32 Da
• Isoelectric Point: 8.2830

>T148842 WP_000854822.1 NZ_CP048107:808438-808821 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Nucleotide

• Length: 384 bp

>T148842 NZ_CP063885:c130025-129693 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13645.39 Da
• Isoelectric Point: 6.4758

>AT148842 WP_048943268.1 NZ_CP048107:807980-808348 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSS
ELNPHRQNTVTLYVKGLTCHADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT148842 NZ_CP063885:c130283-130026 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Toxin

• AlphaFold DB: A0A1M0WJM1