Detailed information of TA system TA148839

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 72030..72828
• Replicon: chromosome
• Accession: NZ_CP048107
• Organism: Escherichia coli strain 201609

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: U9XMP3
• Locus tag: GWK05_RS00300
• Protein ID: WP_000854735.1
• Coordinates: 72030..72407 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A067H947
• Locus tag: GWK05_RS00305
• Protein ID: WP_001285415.1
• Coordinates: 72454..72828 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK05_RS00270	67632..68816	-	1185	WP_096940178.1	sugar efflux transporter	-
GWK05_RS00275	69215..69912	+	698	Protein_54	IS1 family transposase	-
GWK05_RS00280	69977..70123	-	147	WP_001328030.1	hypothetical protein	-
GWK05_RS00285	70391..71233	-	843	WP_000036706.1	DUF4942 domain-containing protein	-
GWK05_RS00290	71330..71527	-	198	WP_000839266.1	DUF957 domain-containing protein	-
GWK05_RS00295	71545..72033	-	489	WP_000761680.1	hypothetical protein	-
GWK05_RS00300	72030..72407	-	378	WP_000854735.1	TA system toxin CbtA family protein	Toxin
GWK05_RS00305	72454..72828	-	375	WP_001285415.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GWK05_RS00310	72908..73129	-	222	WP_000692309.1	DUF987 domain-containing protein	-
GWK05_RS00315	73192..73668	-	477	WP_001366855.1	RadC family protein	-
GWK05_RS00320	73684..74163	-	480	WP_063624909.1	antirestriction protein	-
GWK05_RS00325	74245..75063	-	819	WP_001234688.1	DUF945 domain-containing protein	-
GWK05_RS00330	75162..75395	-	234	WP_001278287.1	DUF905 family protein	-
GWK05_RS00335	75401..76078	-	678	WP_001097301.1	hypothetical protein	-
GWK05_RS00340	76226..76906	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	59888..126619	66731

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14061.07 Da
• Isoelectric Point: 8.2904

>T148839 WP_000854735.1 NZ_CP048107:c72407-72030 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T148839 NZ_CP063884:22427-22843 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13663.32 Da
• Isoelectric Point: 5.8640

>AT148839 WP_001285415.1 NZ_CP048107:c72828-72454 [Escherichia coli]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT148839 NZ_CP063884:22200-22430 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: U9XMP3

Antitoxin

• AlphaFold DB: A0A067H947