Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3172776..3173463 Replicon chromosome
Accession NZ_CP048071
Organism Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag tig002_RS15080 Protein ID WP_003414624.1
Coordinates 3173020..3173463 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag tig002_RS15075 Protein ID WP_003414620.1
Coordinates 3172776..3173033 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
tig002_RS15055 3168096..3168950 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
tig002_RS15060 3169006..3170169 - 1164 WP_031705139.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
tig002_RS15065 3170186..3171400 - 1215 WP_003414610.1 zinc metalloprotease Rip -
tig002_RS15070 3171408..3172649 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
tig002_RS15075 3172776..3173033 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
tig002_RS15080 3173020..3173463 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
tig002_RS15085 3173543..3174205 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
tig002_RS15090 3174301..3174492 + 192 WP_003414632.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
tig002_RS15095 3174824..3176572 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
tig002_RS15100 3176668..3177249 + 582 WP_003414644.1 fasciclin domain-containing protein -
tig002_RS15105 3177349..3177615 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T148830 WP_003414624.1 NZ_CP048071:3173020-3173463 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T148830 NZ_CP063881:c2382636-2382253 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT148830 WP_003414620.1 NZ_CP048071:3172776-3173033 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT148830 NZ_CP063881:c2382878-2382636 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References