Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3126576..3127180 Replicon chromosome
Accession NZ_CP048071
Organism Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag tig002_RS14850 Protein ID WP_003414492.1
Coordinates 3126576..3126968 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag tig002_RS14855 Protein ID WP_003414495.1
Coordinates 3126965..3127180 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
tig002_RS14820 3121726..3122514 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
tig002_RS14825 3122848..3123393 - 546 WP_031705425.1 DUF1802 family protein -
tig002_RS14830 3123665..3124549 - 885 Protein_2911 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
tig002_RS14835 3124552..3125439 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
tig002_RS14840 3125744..3126289 - 546 WP_031705426.1 DUF1802 family protein -
tig002_RS14845 3126286..3126555 - 270 WP_003414489.1 DUF2277 family protein -
tig002_RS14850 3126576..3126968 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
tig002_RS14855 3126965..3127180 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
tig002_RS14860 3127227..3127976 + 750 WP_003414497.1 enoyl-CoA hydratase -
tig002_RS14865 3128055..3129137 - 1083 WP_003414499.1 ABC transporter ATP-binding protein -
tig002_RS14870 3129130..3130440 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
tig002_RS14875 3130443..3131270 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
tig002_RS14880 3131267..3132177 - 911 Protein_2921 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T148828 WP_003414492.1 NZ_CP048071:c3126968-3126576 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T148828 NZ_CP063881:c1899270-1898986 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT148828 WP_003414495.1 NZ_CP048071:c3127180-3126965 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT148828 NZ_CP063881:c1899502-1899260 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References