Detailed information of TA system TA148827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3105524..3106094
• Replicon: chromosome
• Accession: NZ_CP048071
• Organism: Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: tig002_RS14725
• Protein ID: WP_003414166.1
• Coordinates: 3105524..3105880 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: tig002_RS14730
• Protein ID: WP_003901465.1
• Coordinates: 3105864..3106094 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
tig002_RS14705	3100971..3102664	-	1694	Protein_2886	alpha/beta hydrolase family protein	-
tig002_RS14710	3102668..3102994	-	327	WP_003414157.1	hypothetical protein	-
tig002_RS14715	3103167..3103754	+	588	WP_031705109.1	DUF3558 domain-containing protein	-
tig002_RS14720	3103773..3105422	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
tig002_RS14725	3105524..3105880	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
tig002_RS14730	3105864..3106094	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
tig002_RS14735	3106137..3107180	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
tig002_RS14740	3107371..3107646	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
tig002_RS14745	3107822..3108076	-	255	WP_003917684.1	hypothetical protein	-
tig002_RS14750	3108224..3108628	+	405	WP_003414181.1	hypothetical protein	-
tig002_RS14755	3108625..3108816	+	192	WP_003414184.1	hypothetical protein	-
tig002_RS14760	3108880..3110169	+	1290	Protein_2897	transposase family protein	-
tig002_RS14765	3110403..3110660	+	258	WP_003899489.1	hypothetical protein	-
tig002_RS14770	3110765..3110952	+	188	Protein_2899	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T148827 WP_003414166.1 NZ_CP048071:c3105880-3105524 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T148827 NZ_CP063881:c1808662-1808285 [Klebsiella pneumoniae]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGACCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT148827 WP_003901465.1 NZ_CP048071:c3106094-3105864 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT148827 NZ_CP063881:c1809120-1808752 [Klebsiella pneumoniae]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCATGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3