Detailed information of TA system TA148825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3065521..3066200
• Replicon: chromosome
• Accession: NZ_CP048071
• Organism: Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: tig002_RS14500
• Protein ID: WP_003414059.1
• Coordinates: 3065521..3065937 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: tig002_RS14505
• Protein ID: WP_003414061.1
• Coordinates: 3065934..3066200 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
tig002_RS14480	3061573..3062475	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
tig002_RS14485	3062544..3063296	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
tig002_RS14490	3063540..3063815	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
tig002_RS14495	3063812..3065434	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
tig002_RS14500	3065521..3065937	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
tig002_RS14505	3065934..3066200	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
tig002_RS14510	3066226..3066621	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
tig002_RS14515	3066618..3066887	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
tig002_RS14520	3066897..3067991	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
tig002_RS14525	3067988..3068407	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
tig002_RS14530	3068481..3068960	-	480	WP_003414073.1	dihydrofolate reductase	-
tig002_RS14535	3069031..3069831	-	801	WP_003911953.1	thymidylate synthase	-
tig002_RS14540	3069987..3070724	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T148825 WP_003414059.1 NZ_CP048071:c3065937-3065521 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T148825 NZ_CP063881:1157944-1158162 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT148825 WP_003414061.1 NZ_CP048071:c3066200-3065934 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT148825 NZ_CP063881:1157543-1157917 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64