Detailed information of TA system TA148823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2926838..2927552
• Replicon: chromosome
• Accession: NZ_CP048071
• Organism: Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: tig002_RS13690
• Protein ID: WP_003413460.1
• Coordinates: 2927112..2927552 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ20
• Locus tag: tig002_RS13685
• Protein ID: WP_003413456.1
• Coordinates: 2926838..2927125 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
tig002_RS13650	2922260..2922505	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
tig002_RS13655	2922502..2922906	+	405	WP_003413432.1	type II toxin-antitoxin system VapC family toxin	-
tig002_RS13660	2923123..2923743	+	621	WP_003413441.1	DUF4178 domain-containing protein	-
tig002_RS13665	2923754..2924248	+	495	WP_003413444.1	DUF2617 family protein	-
tig002_RS13670	2924245..2924676	+	432	WP_003413445.1	DUF4247 domain-containing protein	-
tig002_RS13675	2924701..2925159	+	459	WP_003413451.1	DUF350 domain-containing protein	-
tig002_RS13680	2925156..2926727	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
tig002_RS13685	2926838..2927125	+	288	WP_003413456.1	antitoxin VapB41	Antitoxin
tig002_RS13690	2927112..2927552	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
tig002_RS13695	2927573..2928328	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
tig002_RS13700	2928461..2929057	-	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
tig002_RS13705	2929065..2929910	-	846	WP_031704959.1	acyl-CoA thioesterase II	-
tig002_RS13710	2929939..2930838	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
tig002_RS13715	2930966..2931640	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T148823 WP_003413460.1 NZ_CP048071:2927112-2927552 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T148823 NZ_CP063880:c30717-30301 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10453.98 Da
• Isoelectric Point: 7.2797

>AT148823 WP_003413456.1 NZ_CP048071:2926838-2927125 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT148823 NZ_CP063880:c30944-30714 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: A0A7U4BWB8