Detailed information of TA system TA148822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2866500..2867137
• Replicon: chromosome
• Accession: NZ_CP048071
• Organism: Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: tig002_RS13425
• Protein ID: WP_003413180.1
• Coordinates: 2866500..2866895 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: tig002_RS13430
• Protein ID: WP_031704849.1
• Coordinates: 2866892..2867137 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
tig002_RS13385	2862586..2863245	+	660	WP_003900846.1	LppA family lipoprotein	-
tig002_RS13390	2863242..2863900	+	659	Protein_2626	hypothetical protein	-
tig002_RS13395	2863897..2864559	+	663	WP_003900848.1	hypothetical protein	-
tig002_RS13400	2864556..2864834	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
tig002_RS13405	2864927..2865340	+	414	WP_003413164.1	PIN domain nuclease	-
tig002_RS13410	2865379..2865636	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
tig002_RS13415	2865633..2866010	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
tig002_RS13420	2866026..2866400	-	375	WP_003413177.1	hypothetical protein	-
tig002_RS13425	2866500..2866895	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
tig002_RS13430	2866892..2867137	-	246	WP_031704849.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
tig002_RS13435	2867548..2867967	-	420	WP_003413190.1	A24 family peptidase	-
tig002_RS13440	2867979..2868788	-	810	WP_003413193.1	shikimate dehydrogenase	-
tig002_RS13445	2868785..2870038	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
tig002_RS13450	2870031..2870543	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T148822 WP_003413180.1 NZ_CP048071:c2866895-2866500 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T148822 NZ_CP063879:c58862-58551 [Klebsiella pneumoniae]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9056.25 Da
• Isoelectric Point: 5.6007

>AT148822 WP_031704849.1 NZ_CP048071:c2867137-2866892 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDGVYGKH
E

Nucleotide

• Length: 246 bp

>AT148822 NZ_CP063879:c58554-58135 [Klebsiella pneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF