Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2865379..2866010 Replicon chromosome
Accession NZ_CP048071
Organism Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag tig002_RS13415 Protein ID WP_003413174.1
Coordinates 2865633..2866010 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag tig002_RS13410 Protein ID WP_003413167.1
Coordinates 2865379..2865636 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
tig002_RS13375 2860638..2860952 + 315 WP_009937839.1 hypothetical protein -
tig002_RS13380 2861248..2862459 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
tig002_RS13385 2862586..2863245 + 660 WP_003900846.1 LppA family lipoprotein -
tig002_RS13390 2863242..2863900 + 659 Protein_2626 hypothetical protein -
tig002_RS13395 2863897..2864559 + 663 WP_003900848.1 hypothetical protein -
tig002_RS13400 2864556..2864834 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
tig002_RS13405 2864927..2865340 + 414 WP_003413164.1 PIN domain nuclease -
tig002_RS13410 2865379..2865636 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
tig002_RS13415 2865633..2866010 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
tig002_RS13420 2866026..2866400 - 375 WP_003413177.1 hypothetical protein -
tig002_RS13425 2866500..2866895 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
tig002_RS13430 2866892..2867137 - 246 WP_031704849.1 type II toxin-antitoxin system antitoxin VapB20 -
tig002_RS13435 2867548..2867967 - 420 WP_003413190.1 A24 family peptidase -
tig002_RS13440 2867979..2868788 - 810 WP_003413193.1 shikimate dehydrogenase -
tig002_RS13445 2868785..2870038 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
tig002_RS13450 2870031..2870543 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T148821 WP_003413174.1 NZ_CP048071:2865633-2866010 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T148821 NZ_CP063878:c5397573-5397256 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT148821 WP_003413167.1 NZ_CP048071:2865379-2865636 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT148821 NZ_CP063878:c5397263-5396976 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References