Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2230443..2231029 Replicon chromosome
Accession NZ_CP048071
Organism Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag tig002_RS10440 Protein ID WP_031704868.1
Coordinates 2230443..2230787 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag tig002_RS10445 Protein ID WP_003410014.1
Coordinates 2230781..2231029 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
tig002_RS10405 2226149..2226748 + 600 WP_003409989.1 L-lysine exporter -
tig002_RS10410 2227164..2227592 + 429 WP_003409992.1 cellulose-binding protein -
tig002_RS10415 2227818..2228357 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
tig002_RS10420 2228877..2229437 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
tig002_RS10425 2229434..2229775 - 342 WP_003410003.1 DUF2384 domain-containing protein -
tig002_RS10430 2229861..2230118 + 258 WP_003410006.1 hypothetical protein -
tig002_RS10435 2230019..2230354 - 336 WP_003410009.1 dehydrogenase -
tig002_RS10440 2230443..2230787 - 345 WP_031704868.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
tig002_RS10445 2230781..2231029 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
tig002_RS10450 2231129..2233444 - 2316 WP_003899120.1 cation transporter ATPase CptG -
tig002_RS10455 2233441..2233713 - 273 WP_003410017.1 DUF1490 family protein -
tig002_RS10460 2233766..2234122 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
tig002_RS10465 2234279..2235046 + 768 WP_080698898.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12258.18 Da        Isoelectric Point: 9.8887

>T148812 WP_031704868.1 NZ_CP048071:c2230787-2230443 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPVTTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T148812 NZ_CP063878:377730-378107 [Klebsiella pneumoniae]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACGCTTAACGACACGCCGTTTGGCAACGACGGTGTGATTCAGGAGCATATCG
ACGCCGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAGAAGTACGATCTGGTGCGCATCGATCGTCACGGTTTC
AGTACAGAAACACAGTTGCCGCGTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGTTGATGACTCGCAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT148812 WP_003410014.1 NZ_CP048071:c2231029-2230781 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT148812 NZ_CP063878:377320-377679 [Klebsiella pneumoniae]
TTGTCGAATAAAACACCGATAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTGCAGGCGGGCAACTGTCTGCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACG
CGGTTTTACGGCATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCACT
CCCCGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References