Detailed information of TA system TA148812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2230443..2231029
• Replicon: chromosome
• Accession: NZ_CP048071
• Organism: Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: tig002_RS10440
• Protein ID: WP_031704868.1
• Coordinates: 2230443..2230787 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL58
• Locus tag: tig002_RS10445
• Protein ID: WP_003410014.1
• Coordinates: 2230781..2231029 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
tig002_RS10405	2226149..2226748	+	600	WP_003409989.1	L-lysine exporter	-
tig002_RS10410	2227164..2227592	+	429	WP_003409992.1	cellulose-binding protein	-
tig002_RS10415	2227818..2228357	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
tig002_RS10420	2228877..2229437	-	561	WP_003410001.1	RES family NAD+ phosphorylase	-
tig002_RS10425	2229434..2229775	-	342	WP_003410003.1	DUF2384 domain-containing protein	-
tig002_RS10430	2229861..2230118	+	258	WP_003410006.1	hypothetical protein	-
tig002_RS10435	2230019..2230354	-	336	WP_003410009.1	dehydrogenase	-
tig002_RS10440	2230443..2230787	-	345	WP_031704868.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	Toxin
tig002_RS10445	2230781..2231029	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	Antitoxin
tig002_RS10450	2231129..2233444	-	2316	WP_003899120.1	cation transporter ATPase CptG	-
tig002_RS10455	2233441..2233713	-	273	WP_003410017.1	DUF1490 family protein	-
tig002_RS10460	2233766..2234122	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-
tig002_RS10465	2234279..2235046	+	768	WP_080698898.1	hemerythrin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-38)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12258.18 Da
• Isoelectric Point: 9.8887

>T148812 WP_031704868.1 NZ_CP048071:c2230787-2230443 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPVTTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Nucleotide

• Length: 345 bp

>T148812 NZ_CP063878:377730-378107 [Klebsiella pneumoniae]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACGCTTAACGACACGCCGTTTGGCAACGACGGTGTGATTCAGGAGCATATCG
ACGCCGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAGAAGTACGATCTGGTGCGCATCGATCGTCACGGTTTC
AGTACAGAAACACAGTTGCCGCGTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGTTGATGACTCGCAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9290.34 Da
• Isoelectric Point: 4.3757

>AT148812 WP_003410014.1 NZ_CP048071:c2231029-2230781 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Nucleotide

• Length: 249 bp

>AT148812 NZ_CP063878:377320-377679 [Klebsiella pneumoniae]
TTGTCGAATAAAACACCGATAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTGCAGGCGGGCAACTGTCTGCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACG
CGGTTTTACGGCATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCACT
CCCCGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U4FAW9