Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mbcAT/RES-TIGR02293
Location 2228877..2229775 Replicon chromosome
Accession NZ_CP048071
Organism Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)


Gene name mbcT Uniprot ID P64908
Locus tag tig002_RS10420 Protein ID WP_003410001.1
Coordinates 2228877..2229437 (-) Length 187 a.a.

Antitoxin (Protein)


Gene name mbcA Uniprot ID P64910
Locus tag tig002_RS10425 Protein ID WP_003410003.1
Coordinates 2229434..2229775 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
tig002_RS10390 2224046..2224699 - 654 WP_003409976.1 cutinase Cfp21 -
tig002_RS10395 2224814..2225044 + 231 WP_003409980.1 DUF167 domain-containing protein -
tig002_RS10400 2225129..2226040 - 912 WP_031704867.1 ArgP/LysG family DNA-binding transcriptional regulator -
tig002_RS10405 2226149..2226748 + 600 WP_003409989.1 L-lysine exporter -
tig002_RS10410 2227164..2227592 + 429 WP_003409992.1 cellulose-binding protein -
tig002_RS10415 2227818..2228357 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
tig002_RS10420 2228877..2229437 - 561 WP_003410001.1 RES family NAD+ phosphorylase Toxin
tig002_RS10425 2229434..2229775 - 342 WP_003410003.1 DUF2384 domain-containing protein Antitoxin
tig002_RS10430 2229861..2230118 + 258 WP_003410006.1 hypothetical protein -
tig002_RS10435 2230019..2230354 - 336 WP_003410009.1 dehydrogenase -
tig002_RS10440 2230443..2230787 - 345 WP_031704868.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 -
tig002_RS10445 2230781..2231029 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family -
tig002_RS10450 2231129..2233444 - 2316 WP_003899120.1 cation transporter ATPase CptG -
tig002_RS10455 2233441..2233713 - 273 WP_003410017.1 DUF1490 family protein -
tig002_RS10460 2233766..2234122 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(25-148)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(69-110)


Sequences

Toxin        


Download         Length: 187 a.a.        Molecular weight: 20248.70 Da        Isoelectric Point: 4.5091

>T148811 WP_003410001.1 NZ_CP048071:c2229437-2228877 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Download         Length: 561 bp

>T148811 NZ_CP063878:125184-125402 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12488.33 Da        Isoelectric Point: 4.8359

>AT148811 WP_003410003.1 NZ_CP048071:c2229775-2229434 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Download         Length: 342 bp

>AT148811 NZ_CP063878:124783-125157 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FKG

Antitoxin

Source ID Structure
PDB 6FKG
AlphaFold DB A0A7U4FAE4

References