Detailed information of TA system TA148810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2221552..2222240
• Replicon: chromosome
• Accession: NZ_CP048071
• Organism: Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0A653
• Locus tag: tig002_RS10375
• Protein ID: WP_003409958.1
• Coordinates: 2221552..2221971 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ28
• Locus tag: tig002_RS10380
• Protein ID: WP_003409968.1
• Coordinates: 2221980..2222240 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
tig002_RS10355	2217047..2217895	+	849	WP_003409942.1	class I SAM-dependent methyltransferase	-
tig002_RS10360	2217858..2219303	-	1446	WP_003409946.1	APC family permease	-
tig002_RS10365	2219482..2220168	-	687	WP_003409954.1	immunoprotective protein Mpt64	-
tig002_RS10370	2220359..2221327	-	969	WP_003899116.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
tig002_RS10375	2221552..2221971	-	420	WP_003409958.1	type II toxin-antitoxin system VapC family toxin	Toxin
tig002_RS10380	2221980..2222240	-	261	WP_003409968.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
tig002_RS10385	2222383..2224058	+	1676	Protein_2036	PecA family PE domain-processing aspartic protease	-
tig002_RS10390	2224046..2224699	-	654	WP_003409976.1	cutinase Cfp21	-
tig002_RS10395	2224814..2225044	+	231	WP_003409980.1	DUF167 domain-containing protein	-
tig002_RS10400	2225129..2226040	-	912	WP_031704867.1	ArgP/LysG family DNA-binding transcriptional regulator	-
tig002_RS10405	2226149..2226748	+	600	WP_003409989.1	L-lysine exporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14724.89 Da
• Isoelectric Point: 7.9535

>T148810 WP_003409958.1 NZ_CP048071:c2221971-2221552 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Nucleotide

• Length: 420 bp

>T148810 NZ_CP063877:c81074-80604 [Klebsiella quasipneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9528.88 Da
• Isoelectric Point: 5.1007

>AT148810 WP_003409968.1 NZ_CP048071:c2222240-2221980 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Nucleotide

• Length: 261 bp

>AT148810 NZ_CP063877:c81514-81071 [Klebsiella quasipneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAAGTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACTGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A829C4H9

Antitoxin

• AlphaFold DB: A0A829C483