Detailed information of TA system TA148807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2186303..2187006
• Replicon: chromosome
• Accession: NZ_CP048071
• Organism: Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: tig002_RS10130
• Protein ID: WP_003409778.1
• Coordinates: 2186303..2186632 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: tig002_RS10135
• Protein ID: WP_003409780.1
• Coordinates: 2186629..2187006 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
tig002_RS10110	2182686..2183756	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
tig002_RS10115	2183753..2184268	+	516	WP_003409718.1	flavin reductase family protein	-
tig002_RS10120	2184265..2185326	+	1062	WP_031705469.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
tig002_RS10125	2185323..2186093	+	771	WP_003409775.1	SDR family oxidoreductase	-
tig002_RS10130	2186303..2186632	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
tig002_RS10135	2186629..2187006	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
tig002_RS10140	2187003..2187593	-	591	WP_003910477.1	SEC-C domain-containing protein	-
tig002_RS10145	2187648..2189012	+	1365	WP_031650091.1	HNH endonuclease	-
tig002_RS10150	2189167..2189619	-	453	WP_003899095.1	lipoprotein	-
tig002_RS10155	2189683..2190084	+	402	WP_003409869.1	hypothetical protein	-
tig002_RS10160	2190077..2190259	-	183	WP_003409870.1	hypothetical protein	-
tig002_RS10165	2190373..2190723	-	351	WP_003409871.1	hypothetical protein	-
tig002_RS10170	2190734..2191636	-	903	WP_031705400.1	hypothetical protein	-
tig002_RS10175	2191657..2191848	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T148807 WP_003409778.1 NZ_CP048071:c2186632-2186303 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T148807 NZ_CP063876:12523-12939 [Klebsiella quasipneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT148807 WP_003409780.1 NZ_CP048071:c2187006-2186629 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT148807 NZ_CP063876:12296-12526 [Klebsiella quasipneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8