Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1690853..1691469 Replicon chromosome
Accession NZ_CP048071
Organism Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag tig002_RS07925 Protein ID WP_003407593.1
Coordinates 1691152..1691469 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag tig002_RS07920 Protein ID WP_003900349.1
Coordinates 1690853..1691155 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
tig002_RS07910 1686739..1688586 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
tig002_RS07915 1688587..1690839 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
tig002_RS07920 1690853..1691155 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
tig002_RS07925 1691152..1691469 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
tig002_RS07930 1691466..1692470 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
tig002_RS07935 1692523..1693812 + 1290 WP_003407599.1 serine hydrolase -
tig002_RS07940 1693885..1694643 - 759 WP_205852885.1 methyltransferase domain-containing protein -
tig002_RS07945 1694701..1694913 - 213 WP_003898900.1 dodecin family protein -
tig002_RS07950 1694923..1695147 - 225 WP_160522526.1 NUDIX domain-containing protein -
tig002_RS07955 1695417..1696445 + 1029 WP_169035316.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T148803 WP_003407593.1 NZ_CP048071:1691152-1691469 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T148803 NZ_CP063874:3517174-3517560 [Klebsiella quasipneumoniae]
ATGCAAACGAAATTACCCTTCATGCGGGTGGCATCGTCACGTCCGTCTCCCGTGGGCGTCTGGCAGACCCTGCTGACCTG
CCTGCTGGCACACCACTATGGCCTTACGCTTAATGACACGCCGTTCAGTGATGAACAGGTAATTCAGCAACATATTGATG
CCGGTATCTCGCTGGTTGATGCCCTGAATTTCATTGTTGAAAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTCAGC
ATCAGGGAGCAGTCTCCGTTTATCACACCCATAGATATCCTCCGGGCCAGAAAGGCTACAGGGCTGATGAACCGAGGCAC
GTATAAAGAGGTGACGGCTATTACCAGAGGACAACATCCGCAGGCCAACGCTTCAGGCAAGCGATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT148803 WP_003900349.1 NZ_CP048071:1690853-1691155 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT148803 NZ_CP063874:3516759-3517118 [Klebsiella quasipneumoniae]
TTGTCGAAGAAAACCACGATAGCAACGCATGATATCAGCGAATCCTGGTGGGGGCTCAGACGCAGTGTCTCCGCATGTTT
TGGTGCCCGTCTGGTACAGGAGGGGAACCGCCTGCACTACCTGGCTGACCGCGCGAACTTTGATGGTCAGTTCTGCGATG
CGGATTTGCGCCATCTTGACCAGTCTTTCCCTGTATTGATGAAACAGCTGGAGTTATTGCTCACCAGCGGTGAACTGAAT
CCCCGTCATCAGCACTGCGTCACGTTGTATGCAAAAGGTCTGACCTGCGAGGCCGATACGCTCGGTTCGCATGGCTACGT
TTATATCGCGATTTATTCCACTCCTATAGCCACAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References