Detailed information of TA system TA148802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1578341..1578996
• Replicon: chromosome
• Accession: NZ_CP048071
• Organism: Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7U4FAR1
• Locus tag: tig002_RS07405
• Protein ID: WP_003407268.1
• Coordinates: 1578341..1578742 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: tig002_RS07410
• Protein ID: WP_031705661.1
• Coordinates: 1578739..1578996 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
tig002_RS07390	1573813..1575198	-	1386	WP_003407260.1	cytochrome P450	-
tig002_RS07395	1575276..1576310	+	1035	WP_003407264.1	AraC family transcriptional regulator	-
tig002_RS07400	1576356..1578086	-	1731	WP_169035314.1	PE family protein	-
tig002_RS07405	1578341..1578742	-	402	WP_003407268.1	type II toxin-antitoxin system VapC family toxin	Toxin
tig002_RS07410	1578739..1578996	-	258	WP_031705661.1	ribbon-helix-helix domain-containing protein	Antitoxin
tig002_RS07415	1579079..1580038	-	960	WP_003407276.1	alpha/beta hydrolase	-
tig002_RS07420	1580063..1581025	-	963	WP_003407279.1	alpha/beta hydrolase	-
tig002_RS07425	1581024..1581761	+	738	WP_031647000.1	lysoplasmalogenase	-
tig002_RS07430	1581842..1583809	+	1968	WP_003407285.1	primosomal protein N'	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-108)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-39)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14894.39 Da
• Isoelectric Point: 11.6897

>T148802 WP_003407268.1 NZ_CP048071:c1578742-1578341 [Mycobacterium tuberculosis]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Nucleotide

• Length: 402 bp

>T148802 NZ_CP063874:3482822-3483232 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCTCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GACTGGGAGATCCTCGCGACGCCGTGGGTTATCAACAGCGGAATGCTGCTGCGTTTGCGCCATGTCGATACCCGACGCGG
TCAGCATCTGTGGCTGGCGGCGGACAGCATGGATGCCGGAGAGTGGCGGGACCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9473.61 Da
• Isoelectric Point: 6.4631

>AT148802 WP_031705661.1 NZ_CP048071:c1578996-1578739 [Mycobacterium tuberculosis]
MKRTNIYLDEEQTASLDKLATQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Nucleotide

• Length: 258 bp

>AT148802 NZ_CP063874:3482575-3482841 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TATTCAACTGGCTGATGAACCATGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FAR1