Detailed information of TA system TA148801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PhRel-ATphRel/-
• Location: 1543977..1545027
• Replicon: chromosome
• Accession: NZ_CP048071
• Organism: Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)

• Gene name: phRel
• Uniprot ID: G0TIH2
• Locus tag: tig002_RS07255
• Protein ID: WP_003407164.1
• Coordinates: 1543977..1544798 (+)
• Length: 274 a.a.

Antitoxin (Protein)

• Gene name: ATphRel
• Uniprot ID: -
• Locus tag: tig002_RS07260
• Protein ID: WP_031705625.1
• Coordinates: 1544791..1545027 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
tig002_RS07235	1539535..1540197	-	663	WP_003407152.1	membrane protein	-
tig002_RS07240	1540194..1540979	-	786	WP_003407157.1	membrane protein	-
tig002_RS07245	1541270..1543231	-	1962	WP_003407159.1	sigma-F factor regulator	-
tig002_RS07250	1543370..1543756	-	387	WP_003898845.1	anti-sigma-F factor antagonist RsfA	-
tig002_RS07255	1543977..1544798	+	822	WP_003407164.1	GTP pyrophosphokinase family protein	Toxin
tig002_RS07260	1544791..1545027	+	237	WP_031705625.1	hypothetical protein	Antitoxin
tig002_RS07265	1545099..1546304	-	1206	WP_003918234.1	serine hydrolase	-
tig002_RS07270	1546607..1547392	+	786	WP_003407180.1	lipoprotein LprF	-
tig002_RS07275	1547588..1549057	+	1470	WP_003900326.1	fatty acid desaturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (76-174)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 274 a.a.
• Molecular weight: 30100.10 Da
• Isoelectric Point: 5.8187

>T148801 WP_003407164.1 NZ_CP048071:1543977-1544798 [Mycobacterium tuberculosis]
VVVALVGSAIVDLHSRPPWSNNAVRRLGVALRDGVDPPVDCPSYAEVMLWHADLAAEVQDRIEGRSWSASELLVTSRAKS
QDTLLAKLRRRPYLQLNTIQDIAGVRIDADLLLGEQTRLAREIADHFGADQPAIHDLRDHPHAGYRAVHVWLRLPAGRVE
IQIRTILQSLWANFYELLADAYGRGIRYDERPEQLAAGVVPAQLQELVGVMQDASADLAMHEAEWQHCAEIEYPGQRAMA
LGEASKNKATVLATTKFRLERAINEAESAGGGG

Nucleotide

• Length: 822 bp

>T148801 NZ_CP063874:c2071778-2071458 [Klebsiella quasipneumoniae]
ATGCACATTTCAACTGTACCGGCCAAGGTGCCGGTTTCGTCACGTCTGTCGCCCGTGCAGGTCTGGCAGCAACTTTTAAC
GTATCTGCTGGGTCACCATTACGGCCTGACGCTTAACGACACACCATTCCATGACGACGCGGCCATCGAAGAGCATATCG
ATGCAGGAATCACGCTCGCCGATGCTGTTAATTTTCTGGTGGAACGTTATGAACTGGTACGCATCGACCGCAAAGGGTTT
AGCTGGCAGGAACAGACTCCGTTCCTGACCGCGACTGATATTCTCCGAGCCAGGCGAGCTACCGGATTAATAAATACTTA
A

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8758.84 Da
• Isoelectric Point: 8.5691

>AT148801 WP_031705625.1 NZ_CP048071:1544791-1545027 [Mycobacterium tuberculosis]
VGEVAGYVVGYNRRTHVRRITEFATPQEAMEHRLKLEAERTDSNIEIVALVSKSLGTLKQTHSRYFTGEELNVGNGAR

Nucleotide

• Length: 237 bp

>AT148801 NZ_CP063874:c2072138-2071815 [Klebsiella quasipneumoniae]
ATGTCAATCACACCACCTCATGAATGGGGTCTACAAAGCGATATCACCCCTCGATTTGGCGCTAGATTAGTTCATGAAGG
CAACCGGCTGTATTACCTGGCTGACAGGGCTGGATTGACGGGAAGTTTTAGCCCAATGCACCTGCAGAAAATAGATCTGG
CTTTCCCGCTGTTTGTCGAACAACTGGAAGATATGCTGCATGCTGGCGAACTAAATCCACATCAGCAATGTCAGGTGAAA
ATTCAACTTGCGAGTTTAACCTGTATAGCTGATACCCGTGGTAGCTGTGGTTACGTTTACATCTCTATTTATCCCAAACC
GTAA

Structures

Toxin

• AlphaFold DB: G0TIH2