Detailed information of TA system TA148798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1241939..1242507
• Replicon: chromosome
• Accession: NZ_CP048071
• Organism: Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: tig002_RS05905
• Protein ID: WP_003405865.1
• Coordinates: 1242133..1242507 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: tig002_RS05900
• Protein ID: WP_003405863.1
• Coordinates: 1241939..1242136 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
tig002_RS05880	1237980..1238618	-	639	WP_003898730.1	lipid droplet-associated protein	-
tig002_RS05885	1238708..1239715	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
tig002_RS05890	1239732..1240715	-	984	WP_003898731.1	hypothetical protein	-
tig002_RS05895	1240778..1241851	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
tig002_RS05900	1241939..1242136	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
tig002_RS05905	1242133..1242507	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
tig002_RS05910	1242710..1243408	+	699	WP_003898733.1	hypothetical protein	-
tig002_RS05915	1243526..1243711	+	186	WP_003901093.1	hypothetical protein	-
tig002_RS05920	1243638..1243913	-	276	WP_003405867.1	hypothetical protein	-
tig002_RS05925	1244156..1244479	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
tig002_RS05930	1244494..1245204	-	711	WP_205410584.1	guanylate cyclase	-
tig002_RS20595	1245226..1245435	+	210	WP_003911400.1	hypothetical protein	-
tig002_RS05935	1245387..1246094	-	708	Protein_1162	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T148798 WP_003405865.1 NZ_CP048071:1242133-1242507 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T148798 NZ_CP063874:c1322506-1322189 [Klebsiella quasipneumoniae]
ATGTTCAGAATGGTTGTGCATGTGGACGTTAAAAAGGAGTTGCAGGCGCTTCCGGCTATTGTCCAGGCGAAGATGATCAG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGATAGTAAACCGCTTGGACAAGGGCTATTTGAAA
TTCGAGCGTTAGGTTCGGTACAGGGGCGGGGGATCTACGTTTTTCAGCAAAACAAAACGCTCTTTCTGCTACGTGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTTATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT148798 WP_003405863.1 NZ_CP048071:1241939-1242136 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT148798 NZ_CP063874:c1322196-1321909 [Klebsiella quasipneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAGCACCGATTACGTACTATGCTGGCACAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTTGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCACCAGCCTGGATACCTTAACGCGA
TACGCGTTAGCGTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07