Detailed information of TA system TA148797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1233183..1233814
• Replicon: chromosome
• Accession: NZ_CP048071
• Organism: Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A7U4F9D0
• Locus tag: tig002_RS05850
• Protein ID: WP_003405820.1
• Coordinates: 1233183..1233494 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TGZ7
• Locus tag: tig002_RS05855
• Protein ID: WP_003405836.1
• Coordinates: 1233494..1233814 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
tig002_RS05830	1228664..1230088	-	1425	WP_003405805.1	class II fumarate hydratase	-
tig002_RS05835	1230119..1231207	-	1089	WP_080698903.1	class II fructose-bisphosphatase	-
tig002_RS05840	1231263..1231907	+	645	WP_003915494.1	DUF4245 domain-containing protein	-
tig002_RS05845	1231914..1233071	-	1158	WP_003898727.1	AI-2E family transporter	-
tig002_RS05850	1233183..1233494	-	312	WP_003405820.1	type II toxin-antitoxin system toxin endoribonuclease MazF3	Toxin
tig002_RS05855	1233494..1233814	-	321	WP_003405836.1	type II toxin-antitoxin system antitoxin MazE3	Antitoxin
tig002_RS05860	1233824..1235349	+	1526	Protein_1146	carboxylesterase/lipase family protein	-
tig002_RS05865	1235367..1236479	-	1113	WP_003405840.1	3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase	-
tig002_RS05870	1236489..1236746	-	258	WP_003405844.1	exodeoxyribonuclease VII small subunit	-
tig002_RS05875	1236736..1237983	-	1248	WP_003405846.1	exodeoxyribonuclease VII large subunit	-
tig002_RS05880	1237980..1238618	-	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-85)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11061.82 Da
• Isoelectric Point: 4.9371

>T148797 WP_003405820.1 NZ_CP048071:c1233494-1233183 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Nucleotide

• Length: 312 bp

>T148797 NZ_CP063872:c142611-142084 [Klebsiella pneumoniae]
GTGATGCAAGAGCTGGCGATAGAGATGATCAACGACGAATTTGACTATGACATAGGCGAATTCGATTGTGGAGAAGAAAC
CTTAAATACATTTTTGAAAGACCATCTCAAGCGGCAGCATGATGGGCAAATTTTAAAAGGTTATGTATTAGTAACCAAAG
AACAGAAGCCTAAGCTGTTAGGCTATTACACATTATCCGGGAGCTGTTTCGAGAGAAAAATGCTCCCGTCAAAAACACAG
CAAAGAAAAATCCCTTATCAGAATGCGCCAAGCATTACTTTGGGACGCTTGGCTATTGACAAATCAATTCAAGGTCAGGG
ATGGGGTGAATTGCTAGTAACTCACGCAATGAATGTCGTCTGGGATGCATCTAAGGCCGTAGGAATATATGGTCTCTTCG
TGGAAGCTCTGAACGATAAAGCTAAGCGCTTCTATACCAGATTAGATTTCATACAGCTCGTTGGTGAGAATAGTAATCTG
CTATTTTACCCGACAAAATCTATTGAAAAAATCTTTACCGAAGAGTAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11394.83 Da
• Isoelectric Point: 5.1236

>AT148797 WP_003405836.1 NZ_CP048071:c1233814-1233494 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Nucleotide

• Length: 321 bp

>AT148797 NZ_CP063872:c142888-142619 [Klebsiella pneumoniae]
ATGTCCGCACTGAGAAAGCAGCGCATAGATCTAAGATTAACTGATGACGACAAAAGCTTAATCGAAGAAGCCGCGGCAAT
GTCAAACCAGACTATTACACAGTTTGTAGTTGCCAGTGCCTCAGAACGTGCAGCGGAAGTGATTGAACAACATCGCAGAA
TGGTTCTAAACGAGGAGTCTTGGAACCTTGTCATGGATGCGATCGCAAACCCTGCTGCGCCGAATGAGCGGCTGAAGCGA
GCAGCGAAGCGTCTACAAAAAATGAGGTAG

Structures

Toxin

• AlphaFold DB: A0A7U4F9D0

Antitoxin

• AlphaFold DB: G0TGZ7