Detailed information of TA system TA148794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1011493..1012112
• Replicon: chromosome
• Accession: NZ_CP048071
• Organism: Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: tig002_RS04790
• Protein ID: WP_003404726.1
• Coordinates: 1011678..1012112 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: tig002_RS04785
• Protein ID: WP_003404724.1
• Coordinates: 1011493..1011672 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
tig002_RS04770	1006966..1008546	+	1581	WP_162859419.1	serine hydrolase	-
tig002_RS04775	1008543..1010936	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
tig002_RS04780	1011209..1011367	+	159	WP_003404720.1	hypothetical protein	-
tig002_RS04785	1011493..1011672	+	180	WP_003404724.1	antitoxin	Antitoxin
tig002_RS04790	1011678..1012112	+	435	WP_003404726.1	SRPBCC family protein	Toxin
tig002_RS04795	1012210..1012983	+	774	WP_003404735.1	VOC family protein	-
tig002_RS04800	1013048..1013497	+	450	WP_003404738.1	hypothetical protein	-
tig002_RS04805	1013632..1013862	-	231	WP_003898642.1	hypothetical protein	-
tig002_RS04810	1014029..1015537	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
tig002_RS04815	1015539..1016777	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T148794 WP_003404726.1 NZ_CP048071:1011678-1012112 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T148794 NZ_CP063872:88882-89364 [Klebsiella pneumoniae]
ATGGGACTGAGGCCTCCGGAACCTCTTACCCCTGAACACAACATTGCTGAGTTTTGTTGTCAGGATCAGGTTCTTTCTGA
GTGGTTGAAGAAAAAGGCGCTGAAGAACCACAGAACGGGCATTTCAAGAGTTTTCGTCGTCTGTGCTGAAAACACGAATA
GGGTAATTGCGTACTACTGTTTGGCATCCGGAAGCGTGCACAGAAACACCGTACCTGGAGCATACCGTCGTAATGCACCA
GAGGCTTTACCGGTCATTGTTCTGGGAAGGCTTGCGGTTGACGCTGCCTGGGCAAGAAAAGGGCTGGGAGCTGCGCTGCT
TAAGGATGCGATATACCGAACAGAACATATTGCAATACAGGTTGGTGTTAGGGCGCTTCTTGTTCACGCCTTAAATGATG
AAGTTAGGGAATTTTACACAAAGTTTGGATTCGAGCCCTCCATTGCCAATGCGCTGACATTATTATTTCCGATCAAAACA
TGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT148794 WP_003404724.1 NZ_CP048071:1011493-1011672 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT148794 NZ_CP063872:88613-88891 [Klebsiella pneumoniae]
ATGAAACCAGACATTCGTGAGGCTCCGATAAACATCCGGGCGAAAACATCTCAACGCGATCTTATCGACATGGCCGCAAA
TCTTGTATCTAAATCACGTACTGATTTTATGCTGGAAGTGGCCTGCCGCGAAGCGCAGGACATCTTGTTAGATCAGCGCT
TGTTTCTGCTGGATGATAATCAGTTTAACGCGTTTGTCGAAGAGCTTGACGCCCCTATGACCCCAGAGCGTCAGGCGCGT
ATTGATAACCTGATGAGCCGCAAATCGCCATGGGACTGA

Structures

Antitoxin

• AlphaFold DB: G0TFQ5