Detailed information of TA system TA148784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 692819..693465
• Replicon: chromosome
• Accession: NZ_CP048071
• Organism: Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: tig002_RS03105
• Protein ID: WP_003403137.1
• Coordinates: 692819..693232 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: tig002_RS03110
• Protein ID: WP_003403139.1
• Coordinates: 693229..693465 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
tig002_RS03085	689094..690452	+	1359	Protein_610	MCE family protein	-
tig002_RS03090	690504..690896	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
tig002_RS03095	690893..691150	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
tig002_RS03100	691333..692568	-	1236	WP_003403128.1	ATP-binding protein	-
tig002_RS03105	692819..693232	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
tig002_RS03110	693229..693465	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
tig002_RS03115	693569..694075	-	507	WP_003900973.1	two component system sensor kinase HK1	-
tig002_RS03120	694189..694719	-	531	WP_003917320.1	two component system sensor kinase HK2	-
tig002_RS03125	694703..695398	-	696	WP_003918099.1	two-component system response regulator TcrA	-
tig002_RS03130	695521..695832	+	312	WP_003403164.1	hypothetical protein	-
tig002_RS03135	695904..696854	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
tig002_RS03140	697095..697679	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
tig002_RS03145	697681..698394	+	714	Protein_622	IS607 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T148784 WP_003403137.1 NZ_CP048071:c693232-692819 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T148784 NZ_CP063871:c2263399-2263016 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT148784 WP_003403139.1 NZ_CP048071:c693465-693229 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT148784 NZ_CP063871:c2263641-2263399 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89