Detailed information of TA system TA148774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 20433..21076
• Replicon: plasmid Ps8079A-p3
• Accession: NZ_CP048069
• Organism: Piscirickettsia salmonis strain Ps-8079A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: GW535_RS18540
• Protein ID: WP_016210974.1
• Coordinates: 20433..20840 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E9LHC5
• Locus tag: GW535_RS18545
• Protein ID: WP_016210972.1
• Coordinates: 20849..21076 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GW535_RS18505	15679..16158	-	480	WP_016212234.1	P27 family phage terminase small subunit	-
GW535_RS18510	16235..16627	-	393	WP_155047322.1	HNH endonuclease	-
GW535_RS18515	16962..17936	-	975	WP_054300271.1	IS30 family transposase	-
GW535_RS18520	18034..18399	-	366	WP_016212235.1	hypothetical protein	-
GW535_RS18525	18552..18881	-	330	WP_036780870.1	hypothetical protein	-
GW535_RS18530	18881..19207	-	327	WP_155047323.1	zinc ribbon domain-containing protein	-
GW535_RS18535	19636..20421	-	786	WP_155047324.1	DNA/RNA non-specific endonuclease	-
GW535_RS18540	20433..20840	-	408	WP_016210974.1	DUF4411 family protein	Toxin
GW535_RS18545	20849..21076	-	228	WP_016210972.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GW535_RS18550	21372..22346	+	975	WP_054300271.1	IS30 family transposase	-
GW535_RS18555	22366..22695	-	330	WP_155047330.1	hypothetical protein	-
GW535_RS18560	22837..23310	-	474	WP_016210960.1	single-stranded DNA-binding protein	-
GW535_RS18565	23312..23596	-	285	WP_036794070.1	hypothetical protein	-
GW535_RS18570	23593..23982	-	390	WP_036780969.1	hypothetical protein	-
GW535_RS18575	23975..24214	-	240	WP_155047325.1	hypothetical protein	-
GW535_RS18580	24211..24558	-	348	WP_155047331.1	hypothetical protein	-
GW535_RS18585	24551..24913	-	363	WP_016210959.1	hypothetical protein	-
GW535_RS18590	24882..25442	-	561	WP_016210973.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..49987	49987

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T148774 WP_016210974.1 NZ_CP048069:c20840-20433 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T148774 NZ_CP063867:4287593-4287940 [Klebsiella variicola]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGGTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGG
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8929.02 Da
• Isoelectric Point: 4.5679

>AT148774 WP_016210972.1 NZ_CP048069:c21076-20849 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDHQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT148774 NZ_CP063867:4287940-4288239 [Klebsiella variicola]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGATCCGCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTTACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: E9LHC5