Detailed information of TA system TA148773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 19132..19738
• Replicon: plasmid Ps8079A-p2
• Accession: NZ_CP048068
• Organism: Piscirickettsia salmonis strain Ps-8079A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: GW535_RS18020
• Protein ID: WP_027242955.1
• Coordinates: 19132..19392 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GW535_RS18025
• Protein ID: WP_036780061.1
• Coordinates: 19385..19738 (+)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GW535_RS18000	15364..15765	+	402	WP_162034490.1	helix-turn-helix domain-containing protein	-
GW535_RS18005	15978..16388	+	411	WP_162034491.1	hypothetical protein	-
GW535_RS18010	16501..17733	+	1233	WP_032126623.1	MFS transporter	-
GW535_RS18015	17910..18884	-	975	WP_054300271.1	IS30 family transposase	-
GW535_RS18020	19132..19392	+	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
GW535_RS18025	19385..19738	+	354	WP_036780061.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GW535_RS18030	20015..20743	+	729	WP_017375910.1	IS6 family transposase	-
GW535_RS18035	20810..21100	-	291	Protein_36	IS6 family transposase	-
GW535_RS18040	21137..22063	-	927	WP_162034492.1	hypothetical protein	-
GW535_RS18045	22205..22933	-	729	WP_017375910.1	IS6 family transposase	-
GW535_RS18050	22963..23577	-	615	WP_162034493.1	prolyl oligopeptidase family serine peptidase	-
GW535_RS18055	23939..24481	-	543	WP_162034494.1	PD40 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..73896	73896

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T148773 WP_027242955.1 NZ_CP048068:19132-19392 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T148773 NZ_CP063867:4261265-4261633 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGCGATTTCTCGCGGCCATATTTTCAATGATGGAAATAAGCGTACGGCACTGTTT
ATTACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAACCCGGACTTCGTCGGCATGAGCGTCGAGGCGGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13504.44 Da
• Isoelectric Point: 5.0200

>AT148773 WP_036780061.1 NZ_CP048068:19385-19738 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQIVKR

Nucleotide

• Length: 354 bp

>AT148773 NZ_CP063867:4261047-4261268 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGACAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATTATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87