Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 51588..52194 Replicon plasmid Ps8942B-p5
Accession NZ_CP048061
Organism Piscirickettsia salmonis strain Ps-8942B

Toxin (Protein)


Gene name hicA Uniprot ID A0A0B8UI87
Locus tag GW537_RS18540 Protein ID WP_027242955.1
Coordinates 51934..52194 (-) Length 87 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7U4WZG4
Locus tag GW537_RS18535 Protein ID WP_016211078.1
Coordinates 51588..51941 (-) Length 118 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GW537_RS18500 47356..47496 + 141 WP_162039123.1 hypothetical protein -
GW537_RS18505 47728..48702 - 975 WP_036771330.1 IS30 family transposase -
GW537_RS18510 48800..49147 - 348 WP_027242952.1 type II toxin-antitoxin system HicB family antitoxin -
GW537_RS18515 49140..49394 - 255 WP_027242953.1 type II toxin-antitoxin system HicA family toxin -
GW537_RS18520 49539..49904 - 366 WP_027242954.1 hypothetical protein -
GW537_RS18525 50101..50328 - 228 Protein_69 hypothetical protein -
GW537_RS18530 50437..51411 - 975 WP_069971681.1 IS30 family transposase -
GW537_RS18535 51588..51941 - 354 WP_016211078.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GW537_RS18540 51934..52194 - 261 WP_027242955.1 type II toxin-antitoxin system HicA family toxin Toxin
GW537_RS18545 52425..53015 + 591 WP_016212329.1 site-specific integrase -
GW537_RS18550 53081..53437 - 357 WP_075278739.1 transposase -
GW537_RS18555 53551..54525 + 975 WP_036771330.1 IS30 family transposase -
GW537_RS18560 54522..54965 - 444 WP_178099494.1 transposase -
GW537_RS20075 55170..55445 - 276 WP_171824216.1 hypothetical protein -
GW537_RS18570 55951..56934 - 984 WP_016211499.1 ParB/RepB/Spo0J family partition protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..114110 114110


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-82)

Antitoxin

Uncharacterised protein family HicB

(53-103)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 9695.31 Da        Isoelectric Point: 10.9468

>T148770 WP_027242955.1 NZ_CP048061:c52194-51934 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Download         Length: 261 bp

>T148770 NZ_CP063867:c3526715-3525840 [Klebsiella variicola]
ATGCAGGTTTTACCCTATGTTGCCGTCGAACGGGAATTTGCGCTGAAAGGTGGCACGGCAATCAATCTCTTTATCCGTGA
TTTCCCACGATTATCGGTGGATATCGACCTCGCCTGGGTGCCCCTGGAAAGCAGAACGATCGCTTTACCTCATATACGGG
ATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAGGCAGGTATGAACGCTGTATTACAAGCTAATCGTTCCGATGAG
ATGCGTGTCGTTGTTACTACCGATAGCGCACAAATAAAGATAGAGGTCTCACCGGTCGCACGGGGAACATTATATCCACC
ACAGGAGCGAGAGGTGGTGGGGGCGGTAGAGGACGTTTTTGGCTATGCCAGCCTTCCGGTGGTTTCGTTGCCCGATCTCT
ACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCATCCCAGAGATTTTTATGATGTGAAATTACTTCTTGATGCGCAG
GAGCTTAACCGTCCCATTTTCAATGGGTTTATCGCGTATTTACTTAGCCATAATCGGCCTCTGGCTGAAGTGCTTAGTCC
TCGCTGGAAAGATATTGCTGAACCCTTTTACCGTGAATTCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGA
CAGCAGTCCCCTACAGGATGATTGATGCGTTAAAATCCTGTTTCACGCAACAGGATATCGATTTTTTGCTGTCATTTAAA
CGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAACACGTATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATAT
TCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAGATAAACTGGAAAAGGTGTTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 118 a.a.        Molecular weight: 13492.39 Da        Isoelectric Point: 5.0200

>AT148770 WP_016211078.1 NZ_CP048061:c51941-51588 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Download         Length: 354 bp

>AT148770 NZ_CP063867:c3527521-3526763 [Klebsiella variicola]
ATGCCATCAAAATTAAACTGGCTGCTGCAAAACAGTGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCGGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACACGCAAAGTGGCTGGTTAAAAAAACGAGGCAACGGCGTATACGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTAGCCGCTCCGGTGTATGTCGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGTCGTTCACACTATCTACAGCTCAAACAAAACCAGTGCTGGTTGAGTATGGAAAATAAAGCGCT
GCTGCCAAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGCAAAAACTGCCGGTGCTGGATGATA
AATACCGCGTAACGCTTGATGTAAAAGGGAAAAGACTCACCGGCAGTGCGCCGGAACTGGCTGCTTACGAGTTGTTGAGC
GCGGTACCGGGGACCCTCTCATTTAACCACGCGGCAGAGCTTTTCCAGGGACTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTGCTTTCTGTCAGCCAGTCCGTGCAGACCAAGCGTTTGTATCTCTTTTTCGCCAGTTTTTACGAGCATGGTTGGC
TCAGACGAATCGATAGCCAAAAAATCGATCTGGGTGCGGGTAAGCGGCAAATCGTGGTAAATGGCAAGTTCAATGCTCAA
TATCAAATCACTGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B8UI87

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4WZG4

References