Detailed information of TA system TA148767

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 100550..101081
• Replicon: plasmid Ps8942B-p4
• Accession: NZ_CP048060
• Organism: Piscirickettsia salmonis strain Ps-8942B

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A7U4YYE1
• Locus tag: GW537_RS17505
• Protein ID: WP_027243212.1
• Coordinates: 100550..100837 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1L6TI86
• Locus tag: GW537_RS17510
• Protein ID: WP_027243211.1
• Coordinates: 100827..101081 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GW537_RS17470	96140..97117	+	978	WP_036771347.1	IS5 family transposase	-
GW537_RS17475	97033..97707	-	675	WP_162039112.1	IS5 family transposase	-
GW537_RS17480	97865..98077	-	213	WP_053093683.1	hypothetical protein	-
GW537_RS17485	98166..98282	+	117	Protein_131	phage minor tail protein L	-
GW537_RS17490	98251..98745	+	495	Protein_132	hypothetical protein	-
GW537_RS17495	99078..100055	+	978	WP_036771347.1	IS5 family transposase	-
GW537_RS17500	100082..100543	+	462	Protein_134	tail assembly protein	-
GW537_RS17505	100550..100837	-	288	WP_027243212.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GW537_RS17510	100827..101081	-	255	WP_027243211.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
GW537_RS17515	101299..101460	-	162	WP_155046636.1	hypothetical protein	-
GW537_RS17520	101475..102452	-	978	WP_036771347.1	IS5 family transposase	-
GW537_RS17525	102549..102737	-	189	Protein_139	hypothetical protein	-
GW537_RS17530	102933..103910	+	978	WP_036771347.1	IS5 family transposase	-
GW537_RS17535	103950..104294	+	345	Protein_141	DDE-type integrase/transposase/recombinase	-
GW537_RS17540	104376..105356	-	981	WP_144420849.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..208102	208102

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (8-55)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11063.21 Da
• Isoelectric Point: 10.8855

>T148767 WP_027243212.1 NZ_CP048060:c100837-100550 [Piscirickettsia salmonis]
MTYKLQFHKEAYKEWKKLNPTTQQQFQKVLKKRLINPHIPSAVCGGDLKGCYKIKLRALGYRLIYTVKKDKVVVQVLAIG
SRNRLEAYRLAASRL

Nucleotide

• Length: 288 bp

>T148767 NZ_CP063867:2603183-2603401 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9554.83 Da
• Isoelectric Point: 4.3176

>AT148767 WP_027243211.1 NZ_CP048060:c101081-100827 [Piscirickettsia salmonis]
MKQLYAQQSVTITELRKSPTSVINEANGEPVVILNHNVPAAYMVPPELFEAMLDSYEDRQLEGIVKKRLHSPDRDFIDVD
VDDL

Nucleotide

• Length: 255 bp

>AT148767 NZ_CP063867:2602782-2603156 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7U4YYE1

Antitoxin

• AlphaFold DB: A0A1L6TI86