Detailed information of TA system TA148759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 6101092..6101681
• Replicon: chromosome
• Accession: NZ_CP048051
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain zm-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: GSF17_RS27530
• Protein ID: WP_124312233.1
• Coordinates: 6101382..6101681 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GSF17_RS27525
• Protein ID: WP_041987449.1
• Coordinates: 6101092..6101385 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSF17_RS27505	6096633..6097796	-	1164	WP_124312230.1	acyltransferase	-
GSF17_RS27510	6097991..6099181	-	1191	WP_124309654.1	serine hydrolase	-
GSF17_RS27515	6099308..6100195	+	888	WP_009051074.1	LysR family transcriptional regulator	-
GSF17_RS27520	6100567..6100959	+	393	WP_162096184.1	hypothetical protein	-
GSF17_RS27525	6101092..6101385	-	294	WP_041987449.1	putative addiction module antidote protein	Antitoxin
GSF17_RS27530	6101382..6101681	-	300	WP_124312233.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GSF17_RS27535	6101852..6102301	+	450	WP_009051078.1	DUF3828 domain-containing protein	-
GSF17_RS27540	6102326..6103336	-	1011	WP_172834887.1	NAD(P)-dependent alcohol dehydrogenase	-
GSF17_RS27545	6103394..6103693	-	300	WP_009051080.1	ArsR family transcriptional regulator	-
GSF17_RS27550	6103757..6104848	-	1092	WP_162096185.1	nitrite reductase, copper-containing	-
GSF17_RS27555	6105074..6105316	+	243	WP_007924102.1	exodeoxyribonuclease VII small subunit	-
GSF17_RS27560	6105313..6106200	+	888	WP_038575447.1	(2E,6E)-farnesyl diphosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11237.99 Da
• Isoelectric Point: 10.3437

>T148759 WP_124312233.1 NZ_CP048051:c6101681-6101382 [Pseudomonas chlororaphis subsp. aurantiaca]
MIEIKQTATFMEWENRLKDSRARALIAARIFRLANGLAGDVSPVGEGVSELRIHYGPGYRVYFQQCGLQLVILLCGGDKS
RQSRDIELAKQLARRWSRS

Nucleotide

• Length: 300 bp

>T148759 NZ_CP063865:3861235-3861768 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATAAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10446.94 Da
• Isoelectric Point: 5.6558

>AT148759 WP_041987449.1 NZ_CP048051:c6101385-6101092 [Pseudomonas chlororaphis subsp. aurantiaca]
MNEPLFDYDPATSLRDPQAIALFMADAFETGDAGYIAKALGVVARAKGMTELAHETGLSREQLYRSFSDKGNPTLKTLLA
VMRALGLDMTARVHGAS

Nucleotide

• Length: 294 bp

>AT148759 NZ_CP063865:3860958-3861224 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA