Detailed information of TA system TA148757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2396250..2396835
• Replicon: chromosome
• Accession: NZ_CP048051
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain zm-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7S5HMS4
• Locus tag: GSF17_RS11090
• Protein ID: WP_016705075.1
• Coordinates: 2396542..2396835 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GSF17_RS11085
• Protein ID: WP_162094885.1
• Coordinates: 2396250..2396540 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSF17_RS11075	2393762..2395144	+	1383	WP_162094884.1	efflux transporter outer membrane subunit	-
GSF17_RS11080	2395263..2395850	+	588	WP_124308120.1	hypothetical protein	-
GSF17_RS11085	2396250..2396540	-	291	WP_162094885.1	putative addiction module antidote protein	Antitoxin
GSF17_RS11090	2396542..2396835	-	294	WP_016705075.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GSF17_RS11095	2397070..2398464	-	1395	WP_162094886.1	VOC family protein	-
GSF17_RS11100	2398729..2399853	+	1125	WP_052107416.1	diguanylate cyclase	-
GSF17_RS11105	2399947..2401191	+	1245	WP_162094887.1	M20/M25/M40 family metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-97)

Antitoxin

• Uncharacterized protein HI1420 (8-94)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10699.27 Da
• Isoelectric Point: 10.0959

>T148757 WP_016705075.1 NZ_CP048051:c2396835-2396542 [Pseudomonas chlororaphis subsp. aurantiaca]
MDYEILQTTVFAQWHTTLRDLRARIAIARRIERASAGNLGDVKNLGGGLSEMRVNTGAGYRLYFTVRGNTLIVLLLGGDK
SSQPADICQARSLAKEF

Nucleotide

• Length: 294 bp

>T148757 NZ_CP063865:3735333-3735617 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGTGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10297.81 Da
• Isoelectric Point: 6.9755

>AT148757 WP_162094885.1 NZ_CP048051:c2396540-2396250 [Pseudomonas chlororaphis subsp. aurantiaca]
MSDTLVPFDIAALLDSDQAISEYLSQVLADDDTDELIRALGHVARARGMTQIAKTTGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVSRG

Nucleotide

• Length: 291 bp

>AT148757 NZ_CP063865:3735101-3735343 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGTGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A7S5HMS4