Detailed information of TA system TA148739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 41668..42269
• Replicon: plasmid pTET-GZEC065
• Accession: NZ_CP048027
• Organism: Escherichia coli strain GZEC065

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: GWG09_RS23955
• Protein ID: WP_033551393.1
• Coordinates: 41668..42048 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: GWG09_RS23960
• Protein ID: WP_001190712.1
• Coordinates: 42048..42269 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWG09_RS23920	37060..37269	+	210	WP_000874156.1	hypothetical protein	-
GWG09_RS23925	37380..38231	+	852	WP_000611664.1	hypothetical protein	-
GWG09_RS23930	38264..39379	-	1116	WP_033551397.1	phage antirepressor KilAC domain-containing protein	-
GWG09_RS23935	39376..39735	-	360	Protein_47	host cell division inhibitor Icd-like protein	-
GWG09_RS23940	39957..40322	-	366	Protein_48	terminase	-
GWG09_RS23945	40413..41456	-	1044	WP_033551394.1	DUF968 domain-containing protein	-
GWG09_RS23950	41484..41663	-	180	WP_000113018.1	hypothetical protein	-
GWG09_RS23955	41668..42048	-	381	WP_033551393.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GWG09_RS23960	42048..42269	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GWG09_RS23965	42452..42628	+	177	Protein_53	type I restriction-modification system subunit M N-terminal domain-containing protein	-
GWG09_RS23970	42686..43091	+	406	Protein_54	IS1 family transposase	-
GWG09_RS23975	43125..44633	-	1509	Protein_55	Tn3 family transposase	-
GWG09_RS23980	44699..45380	+	682	Protein_56	IS6 family transposase	-
GWG09_RS23985	45406..45546	+	141	WP_001044210.1	hypothetical protein	-
GWG09_RS23990	46032..46769	+	738	WP_001366550.1	recombinase family protein	-
GWG09_RS23995	46766..46990	+	225	WP_000743213.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / fosA3	-	1..88706	88706

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13559.26 Da
• Isoelectric Point: 5.6277

>T148739 WP_033551393.1 NZ_CP048027:c42048-41668 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGGISVSSVADTLRRLYGSSE

Nucleotide

• Length: 381 bp

>T148739 NZ_CP063863:c987357-987073 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTTAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT148739 WP_001190712.1 NZ_CP048027:c42269-42048 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT148739 NZ_CP063863:c987589-987347 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCTGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6