Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4369917..4370535 Replicon chromosome
Accession NZ_CP048025
Organism Escherichia coli strain GZEC065

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GWG09_RS20830 Protein ID WP_001291435.1
Coordinates 4369917..4370135 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID E9TLB8
Locus tag GWG09_RS20835 Protein ID WP_000344794.1
Coordinates 4370161..4370535 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWG09_RS20795 4365207..4365779 + 573 WP_000779831.1 YbaY family lipoprotein -
GWG09_RS20800 4365810..4366121 - 312 WP_000409911.1 MGMT family protein -
GWG09_RS20810 4366500..4366853 + 354 WP_000878140.1 DUF1428 domain-containing protein -
GWG09_RS20815 4366895..4368445 - 1551 WP_001364591.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GWG09_RS20820 4368609..4369079 - 471 WP_000136192.1 YlaC family protein -
GWG09_RS20825 4369195..4369746 - 552 WP_000102564.1 maltose O-acetyltransferase -
GWG09_RS20830 4369917..4370135 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GWG09_RS20835 4370161..4370535 - 375 WP_000344794.1 Hha toxicity modulator TomB Antitoxin
GWG09_RS20840 4371081..4374230 - 3150 WP_001132470.1 multidrug efflux RND transporter permease subunit -
GWG09_RS20845 4374253..4375446 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T148729 WP_001291435.1 NZ_CP048025:c4370135-4369917 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T148729 NZ_CP063858:3376855-3377139 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14527.36 Da        Isoelectric Point: 4.9284

>AT148729 WP_000344794.1 NZ_CP048025:c4370535-4370161 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGRVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT148729 NZ_CP063858:3376623-3376865 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure

References