Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2754507..2755138 Replicon chromosome
Accession NZ_CP048025
Organism Escherichia coli strain GZEC065

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag GWG09_RS13365 Protein ID WP_001259388.1
Coordinates 2754863..2755138 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag GWG09_RS13360 Protein ID WP_000593555.1
Coordinates 2754507..2754866 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWG09_RS13335 2750639..2751583 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
GWG09_RS13340 2751580..2752413 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
GWG09_RS13345 2752413..2753177 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
GWG09_RS13350 2753174..2753980 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
GWG09_RS13355 2753986..2754387 + 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
GWG09_RS13360 2754507..2754866 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GWG09_RS13365 2754863..2755138 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
GWG09_RS13370 2755211..2756335 - 1125 WP_001615305.1 ABC transporter permease -
GWG09_RS13375 2756335..2759070 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
GWG09_RS13380 2759067..2760134 - 1068 WP_000361482.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T148722 WP_001259388.1 NZ_CP048025:c2755138-2754863 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T148722 NZ_CP063857:33877-34182 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT148722 WP_000593555.1 NZ_CP048025:c2754866-2754507 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT148722 NZ_CP063857:33657-33875 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References