Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2127650..2128304 Replicon chromosome
Accession NZ_CP048025
Organism Escherichia coli strain GZEC065

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag GWG09_RS10215 Protein ID WP_000244772.1
Coordinates 2127650..2128057 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GWG09_RS10220 Protein ID WP_000354046.1
Coordinates 2128038..2128304 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWG09_RS10195 2123607..2125340 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
GWG09_RS10200 2125346..2126056 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GWG09_RS10205 2126081..2126977 - 897 WP_000806637.1 site-specific tyrosine recombinase XerD -
GWG09_RS10210 2127089..2127610 + 522 WP_001055874.1 flavodoxin FldB -
GWG09_RS10215 2127650..2128057 - 408 WP_000244772.1 protein YgfX Toxin
GWG09_RS10220 2128038..2128304 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GWG09_RS10225 2128547..2129527 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
GWG09_RS10230 2129723..2130382 - 660 WP_000250274.1 hemolysin III family protein -
GWG09_RS10235 2130546..2130857 - 312 WP_001182945.1 N(4)-acetylcytidine aminohydrolase -
GWG09_RS10240 2130902..2132335 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
GWG09_RS10245 2132392..2133135 - 744 WP_000951941.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T148719 WP_000244772.1 NZ_CP048025:c2128057-2127650 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T148719 NZ_CP063856:4166104-4166472 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT148719 WP_000354046.1 NZ_CP048025:c2128304-2128038 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT148719 NZ_CP063856:4165886-4166107 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References