Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 534315..534953 Replicon chromosome
Accession NZ_CP048025
Organism Escherichia coli strain GZEC065

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GWG09_RS02625 Protein ID WP_000813794.1
Coordinates 534315..534491 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GWG09_RS02630 Protein ID WP_001270285.1
Coordinates 534537..534953 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWG09_RS02605 529934..531148 - 1215 WP_001326689.1 BenE family transporter YdcO -
GWG09_RS02610 531201..531737 + 537 WP_000429153.1 DNA-binding transcriptional regulator SutR -
GWG09_RS02615 531810..533771 + 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GWG09_RS02620 533863..534093 - 231 WP_000494244.1 YncJ family protein -
GWG09_RS02625 534315..534491 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GWG09_RS02630 534537..534953 + 417 WP_001270285.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GWG09_RS02635 535032..536438 + 1407 WP_000760590.1 PLP-dependent aminotransferase family protein -
GWG09_RS02640 536683..537828 + 1146 WP_000034363.1 ABC transporter substrate-binding protein -
GWG09_RS02645 537846..538859 + 1014 WP_000220411.1 ABC transporter ATP-binding protein -
GWG09_RS02650 538860..539801 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 528746..529894 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(88-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T148706 WP_000813794.1 NZ_CP048025:534315-534491 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T148706 NZ_CP063852:c171977-171495 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTTATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15247.61 Da        Isoelectric Point: 4.6115

>AT148706 WP_001270285.1 NZ_CP048025:534537-534953 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT148706 NZ_CP063852:c172231-171965 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References