Detailed information of TA system TA148694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 19036..19624
• Replicon: plasmid pAT205
• Accession: NZ_CP048015
• Organism: Acinetobacter towneri strain 205

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9CKJ9
• Locus tag: GVU25_RS12925
• Protein ID: WP_004647749.1
• Coordinates: 19328..19624 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GVU25_RS12920
• Protein ID: WP_004969304.1
• Coordinates: 19036..19326 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVU25_RS12880	14179..14481	-	303	WP_096902794.1	XRE family transcriptional regulator	-
GVU25_RS12885	14474..14831	-	358	Protein_15	type II toxin-antitoxin system RelE/ParE family toxin	-
GVU25_RS12890	15225..15494	-	270	WP_167846496.1	hypothetical protein	-
GVU25_RS12895	15825..16031	-	207	Protein_17	transposase	-
GVU25_RS12900	16178..16459	-	282	WP_000985610.1	putative addiction module antidote protein	-
GVU25_RS12905	16460..16762	-	303	WP_154320144.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GVU25_RS12910	17012..17221	+	210	WP_004976362.1	hypothetical protein	-
GVU25_RS12915	17471..18925	+	1455	WP_026438532.1	SulP family inorganic anion transporter	-
GVU25_RS12920	19036..19326	-	291	WP_004969304.1	putative addiction module antidote protein	Antitoxin
GVU25_RS12925	19328..19624	-	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GVU25_RS12930	20060..20722	-	663	WP_167846497.1	hypothetical protein	-
GVU25_RS12935	20782..21601	+	820	Protein_25	IS5 family transposase	-
GVU25_RS12940	21735..22019	-	285	WP_148245845.1	mobilization protein	-
GVU25_RS12945	22252..23055	+	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
GVU25_RS12950	23055..23891	+	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
GVU25_RS12960	24059..24325	+	267	WP_052928020.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id / tet(Y) / aph(4)-Ia / aac(3)-IVa / sul2 / tet(X6) / dfrA1 / ant(3'')-Ia / floR	-	1..158867	158867
	-	flank	IS/Tn	-	-	15810..16031	221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11584.25 Da
• Isoelectric Point: 5.8801

>T148694 WP_004647749.1 NZ_CP048015:c19624-19328 [Acinetobacter towneri]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGSGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAVKLSKTLED

Nucleotide

• Length: 297 bp

>T148694 NZ_CP063849:3894245-3894511 [Paludibaculum fermentans]
GTGCGGAACCCGATCACGGGCGCACAGTTCGGGCGCGATGTGAAACTCGCTCGAAAGCGCGGCAAGGACATGGCGAAGTT
GCACGACGTCATCCTGCTGCTGATTGAGGGAAGTCCGTTGCCGTCGCGCTACAAGGACCATACTCTTGGCGGAGAATGGA
AGCACTTCCGCGACTGCCACATCCAACCGGATTGGCTGCTGATCTACAAGATTGAGGGCGAGGACCTTTACCTCGTTCGC
ACGGGAACGCACTCGGATCTGTTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10524.19 Da
• Isoelectric Point: 5.1133

>AT148694 WP_004969304.1 NZ_CP048015:c19326-19036 [Acinetobacter towneri]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSAPRFDTI
NRVVNALGLKLTVQHA

Nucleotide

• Length: 291 bp

>AT148694 NZ_CP063849:3893984-3894241 [Paludibaculum fermentans]
ATCATGTCGACCACGATGATCCACGTTCGCGTGGACGAGAAGACAAAGCAAAGAGCCACGAAGACACTTGCGGCCATGGG
CATCTCCGTATCCGATGCGGTCCGCATGCTGTTGGTCCGGGTGGTGGCGGAGAAGGCGCTGCCCTTTGAAGTGAAGGTCC
CGAACGCGACCACGGTGAAGGCGATGCGCGCTGCCGACAAGGGCCAGGGCAAACGCCAGAAGTCCGGCGATACGCTGTTC
AAAGACCTGGGCATCTAG