Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 33088..33613 Replicon plasmid p3575-NDM-5
Accession NZ_CP048012
Organism Escherichia coli strain RUT3575

Toxin (Protein)


Gene name ccdB Uniprot ID A0A9E1GGX2
Locus tag GW576_RS23685 Protein ID WP_016946795.1
Coordinates 33088..33393 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID -
Locus tag GW576_RS23690 Protein ID WP_023294775.1
Coordinates 33395..33613 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GW576_RS23655 28894..29163 + 270 WP_000339857.1 hypothetical protein -
GW576_RS23660 29520..30386 - 867 WP_004118283.1 replication initiation protein -
GW576_RS23665 30921..31025 + 105 WP_032409716.1 hypothetical protein -
GW576_RS23670 31154..31411 + 258 WP_000764642.1 hypothetical protein -
GW576_RS23675 31469..32245 - 777 WP_049245386.1 site-specific integrase -
GW576_RS23680 32424..32930 - 507 WP_023294776.1 hypothetical protein -
GW576_RS23685 33088..33393 - 306 WP_016946795.1 type II toxin-antitoxin system toxin CcdB Toxin
GW576_RS23690 33395..33613 - 219 WP_023294775.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GW576_RS23695 34207..35181 - 975 WP_001148755.1 sensor domain-containing diguanylate cyclase -
GW576_RS23700 35818..36726 + 909 WP_001372261.1 HNH endonuclease -
GW576_RS23705 37066..37338 - 273 Protein_37 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B / rmtB / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 manC / fcl / gmd / galF 1..93625 93625


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11548.22 Da        Isoelectric Point: 5.6831

>T148687 WP_016946795.1 NZ_CP048012:c33393-33088 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T148687 NZ_CP063845:3007011-3007442 [Gloeobacter morelensis MG652769]
GTGAGGCTGCTGCTGGATACTTGCGTGATTGCCGAGTTGCGCAAGCCGCGTCCCGAGCCCACGGTGGTGGATACCATCGG
CGCTGTTGCCGACGATAATCTGTACCTCAGCGTGCTGACGGTTGGCGAGATTTTCAAGGGGATCGGATTGCTCCCGCCGG
GCACAAAAAAACAAGAGTTGAACCATTGGCTGCTCGGCCTGCAGGTGCAGTTTGCAGAGCGTATTTTGGCAGTTGACGCA
GAAACAGCGGAAATCTGGGGTAAGGGCGTTGCTGCCGCTACACAAGCTGGAGTGACTGTGCCGACGATCGACAGCCTGAT
TGCCGCGACCGCCGCCCGTCACGGTCTAGCAGTTGCTACGCGCAATGTGCGCCACTTTCACAGGTTCGGGGTGAGCGTTG
TAAACCCCTGGGATGGCCAAAATGCCCCGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8376.34 Da        Isoelectric Point: 4.7558

>AT148687 WP_023294775.1 NZ_CP048012:c33613-33395 [Escherichia coli]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFAEENRDW

Download         Length: 219 bp

>AT148687 NZ_CP063845:3006772-3007014 [Gloeobacter morelensis MG652769]
ATGCAGTGGCAACTGGCTGACGCGAAAAACAGATTTAGCGACCTCGTGGAGCGCGCCTTACGGGAAGGTCCTCAAACGGT
GACAAAGCGGGGTATCGAAGCTGTGGTCGTGCTGTCGGCTGAGGAGTACCGTCGATTGTCTGGAGCAAAGCCGAGCTTCA
AGGCTTACTTGATGCAGGGACCGTCCTTCGAGGGACTGGACCTTGAGCGCGATCAAAGTGCTGAGCGGTCTGTAGAACTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References