Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 61989..62632 Replicon plasmid p3575-CTX-M
Accession NZ_CP048011
Organism Escherichia coli strain RUT3575

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag GW576_RS23455 Protein ID WP_001044768.1
Coordinates 62216..62632 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag GW576_RS23450 Protein ID WP_001261287.1
Coordinates 61989..62219 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GW576_RS23440 57167..58300 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
GW576_RS23445 58563..61682 - 3120 WP_023909028.1 hypothetical protein -
GW576_RS23450 61989..62219 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GW576_RS23455 62216..62632 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
GW576_RS23460 62794..64932 - 2139 WP_000350635.1 AAA family ATPase -
GW576_RS23465 65397..66392 + 996 WP_000246636.1 hypothetical protein -
GW576_RS23470 66396..67328 + 933 WP_000991832.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / sul1 / qacE / aadA5 / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr - 1..76937 76937


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T148686 WP_001044768.1 NZ_CP048011:62216-62632 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T148686 NZ_CP063845:c1886824-1886447 [Gloeobacter morelensis MG652769]
ATGATCGCGATCGATACTTCCGCCATCGTCGCCATTCTTCTTGGTGAGCCCGAAGCGGAGCGCTTCGCACGCGCCATCGA
GCAGGACGAAGAACCGCTGCTCTCCGCTGCTTCCCTGGTTGAGCTTTTCCTCGTGATGAAACACAAGCAGGGGTCGGCCT
CTGCGCCCTTGATCGAGCAGTTCCTGGCTACGGGCGGGATCAGCGTCTGTTCTGTCACCCCCGAACAAGCCCGCCGCGCT
CAAGAAGCCGCCTATCGCTTCGGCATACTGAATTTTGGCGATGTGTTTTCTTATGCGCTCGCCAAGGAGAGGGGCATCGG
TCTTCTGTTCAAAGGTACCGACTTCGCGGTGACCGATCTCATCGCCGCACCCTTCTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT148686 WP_001261287.1 NZ_CP048011:61989-62219 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT148686 NZ_CP063845:c1887099-1886821 [Gloeobacter morelensis MG652769]
ATGCAGCAAATCAACATTAAAAGCGACAAAGCCTATCGGATGGCGAACGAACTGAGCCGCTTGACCGGGGAAAGCGTGAC
CCAGGCGATCACCCAAGCCATTGAAGAGCGGCTCGAAAAGATTCGTGCTCAGGCGCGCAGCCGCCGCGAGGGTATCGCCG
AACGACTGCTTGCCCTTGGCACCAGGAGCGTGGCGCTTCCCATCCTTGACCCGCGCGCGCCCGACGAGATCCTCTACGAC
GAGAACGGATTGCCGAAAAACCCGATGGCGGCTCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References