Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 55510..56035 Replicon plasmid p3575-CTX-M
Accession NZ_CP048011
Organism Escherichia coli strain RUT3575

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag GW576_RS23420 Protein ID WP_001159868.1
Coordinates 55510..55815 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A223LLB0
Locus tag GW576_RS23425 Protein ID WP_023909027.1
Coordinates 55817..56035 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GW576_RS23405 51420..52586 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
GW576_RS23410 53174..53929 - 756 WP_000852146.1 replication initiation protein RepE -
GW576_RS23415 54703..55509 - 807 WP_000016982.1 site-specific integrase -
GW576_RS23420 55510..55815 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
GW576_RS23425 55817..56035 - 219 WP_023909027.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GW576_RS23430 56669..56866 + 198 WP_000215657.1 hypothetical protein -
GW576_RS23435 56863..57147 - 285 WP_000642771.1 hypothetical protein -
GW576_RS23440 57167..58300 - 1134 WP_000545983.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / sul1 / qacE / aadA5 / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr - 1..76937 76937


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T148685 WP_001159868.1 NZ_CP048011:c55815-55510 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T148685 NZ_CP063845:c1255476-1255066 [Gloeobacter morelensis MG652769]
ATGAACGCCTACCGCTATTTGCTGGACACCAACATCGTTTCCAATCTCATCCGGTTTCCGGGTGGCTCTGTGGCTGCAAA
TATCGCCCGTATTGGAGAAGCTGGGGTATGCACCAGTATCATCGTCGCCAGTGAACTGCGCTTCGGCGCGGCCAAGAAAG
GCTCGATGCGTCTGTCCGCCCAGCTTGAAACCGTATTGTCGGGGCTTGACGTACTGCCCTTCGAGTTCCCCGGCGATCAG
CACTACGCGACTCTGCGAGCGGCCATCGAAAGGACAGGCACACCGATTGGCTCCAACGACATGTTTATCGCCGCCCACGC
GCTGTACCTTGGATTAATTCTAGTGACCGACAATCTACGCGAATTCGCCCGCATACCGGGGCTGAGCGTCGAGAACTGGC
TTGCGCCCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.31 Da        Isoelectric Point: 4.7405

>AT148685 WP_023909027.1 NZ_CP048011:c56035-55817 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTIQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT148685 NZ_CP063845:c1255714-1255478 [Gloeobacter morelensis MG652769]
ATGCCCACAGAACGTCACGTTCGTCTTTTCAGAAATGGGCGCAATCAGGCTTTGCGCATTCCGCGCGAATTCGAACTGCC
CAGCGAGGAAGCCATCATTCGCAAAGAAGGTGACCGGCTGATTATTGAGCCGCTCAGGCGTCGCTCTTTGCTGGCTCTGC
TTGCAACCTTGGAGCCGCTCGAAGACGAGTTTCCTGACATCGACGATTTACCACCGCTTGACGATGTCAATTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A223LLB0

References