Detailed information of TA system TA148678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 2837885..2839605
• Replicon: chromosome
• Accession: NZ_CP048010
• Organism: Escherichia coli strain RUT3575

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A7U9J2A7
• Locus tag: GW576_RS14000
• Protein ID: WP_000312833.1
• Coordinates: 2837885..2838541 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: B7UP19
• Locus tag: GW576_RS14005
• Protein ID: WP_000634203.1
• Coordinates: 2838538..2839605 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GW576_RS13985	2833800..2834039	+	240	WP_024224214.1	hypothetical protein	-
GW576_RS13990	2834079..2836517	+	2439	WP_032180025.1	type I restriction-modification system subunit M	-
GW576_RS13995	2836529..2837872	+	1344	WP_032180130.1	restriction endonuclease subunit S	-
GW576_RS14000	2837885..2838541	+	657	WP_000312833.1	DUF4433 domain-containing protein	Toxin
GW576_RS14005	2838538..2839605	+	1068	WP_000634203.1	macro domain-containing protein	Antitoxin
GW576_RS14010	2839624..2842719	+	3096	WP_000108736.1	HsdR family type I site-specific deoxyribonuclease	-
GW576_RS14015	2842719..2843435	+	717	WP_001122107.1	M48 family metallopeptidase	-
GW576_RS14020	2843780..2844394	+	615	Protein_2680	IS3-like element IS600 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	blaCMY-2	-	2831145..2866031	34886

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25233.56 Da
• Isoelectric Point: 6.2145

>T148678 WP_000312833.1 NZ_CP048010:2837885-2838541 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Nucleotide

• Length: 657 bp

>T148678 NZ_CP063843:228815-229321 [Morganella morganii]
TTGAACTTAACGGCACCGGAACCCTTGCAGCAGCATCATGATTTTACCGTTTTTCAGTCAGGTGAGGAGTCCCTTAACCA
TTGGCTGAAAACCCGCGCATTGCAAAATCAGCAATCCGGAGCCTCACGGACATTTGTCGTCTGCCACCATAACCGGATAA
TGGCCTATTATGCATTATCGACAGGCGTTATTACCTGTTCACAGGCGGCGGGTCGTTTCAGACGTAATATGCCCCCGGAA
ATTCCGGTTATCCTGCCGGGACGTCTTGCTGTTGACGAAAGCGTAAAAGGCAGAGGGATCGGACGGGGTCTGATAAAAGA
TGCCGCACTGCGGGTCTTGCAGGCGGCCGGGATTGTGGGTATCCGCGGTATTGTTGTCCGTGCTCTGTCTGATAATGCCA
GACGTTTTTATGAGCATACCGGTTTTATGCCGTCACCGGCTGACCCGATGCTGCTGATGATTACATTACGGGATCTGCAG
CTGGCAACCGGTATCTATTCTGAATAG

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40672.70 Da
• Isoelectric Point: 5.6927

>AT148678 WP_000634203.1 NZ_CP048010:2838538-2839605 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT148678 NZ_CP063843:228504-228812 [Morganella morganii]
ATGACAAAACACACAGATGAGCAGCATCCTGTGTCCGGCGTGAATATCACCCGTTCAACACTGAATTTACGCATCAAACC
GGAAGATAAAATTCTGATTGATCGCGCAGCGCATGCTGCCGGTAAAAACAGAACCGAGTTCGTACTGGAAACTGCGCGGC
GTGCCGCCGAAGAAACACTGGCAGATTTACGGGTTATTGATGTCAGTACCGATGTCTATCAGCAGTTTATTACCAGACTG
GATATGGCACCACAGTCCGGTGAGGCGCTGAAGAAGACCATGCTGACGAAGGCGCCGTGGGAGAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9J2A7

Antitoxin

• AlphaFold DB: A0A7U9J2B9